Buchholz H, Schönrock C, Fehr S, Richter D
Institut für Zellbiochemie und klinische Neurobiologie, UKE, Universität Hamburg, Germany.
Mol Mar Biol Biotechnol. 1995 Jun;4(2):179-84.
The nucleotide sequence of a cDNA encoding an isotocin hormone precursor has been elucidated by analyzing a lambda ZAPII library constructed using poly(A)+ RNA from the brain of the cartilaginous fish Torpedo marmorata. The sequence predicts a precursor of 126 amino acid residues that consists of a signal peptide, the isotocin moiety, and a neurophysin carrier protein. In contrast to other known fish isotocin precursor sequences, the Torpedo neurophysin moiety is not extended at its carboxy-terminus by a copeptin-like sequence. The T. marmorata isotocin precursor exhibits highest amino acid sequence identity (61%) to the toad mesotocin precursor. As demonstrated by in situ hybridization, the isotocin mRNA is present in neurons of the preoptic area of the Torpedo brain.
通过分析使用来自软骨鱼电鳐大脑的聚腺苷酸加尾RNA构建的λZAPII文库，已阐明了编码异催产素激素前体的cDNA的核苷酸序列。该序列预测了一个由126个氨基酸残基组成的前体，它由一个信号肽、异催产素部分和一个神经垂体素载体蛋白组成。与其他已知的鱼类异催产素前体序列不同，电鳐的神经垂体素部分在其羧基末端没有被类似 copeptin的序列延长。电鳐异催产素前体与蟾蜍中催产素前体表现出最高的氨基酸序列同一性（61%）。原位杂交表明，异催产素mRNA存在于电鳐大脑视前区的神经元中。
