Tanhuanpää P K, Vilkki J P, Vilkki H J
Plant Breeding Section, Institute of Crop and Soil Science, Agricultural Research Centre of Finland, Jokioinen.
Genome. 1995 Apr;38(2):414-6. doi: 10.1139/g95-053.
The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 +/- 1.35% and in those lacking the marker was 5.70 +/- 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.
以油菜品种托帕斯（Brassica napus L.）与R4（低亚麻酸突变系）杂交得到的F2代（64个个体）为材料，利用8个RFLP标记和34个RAPD标记进行分析，以寻找影响亚麻酸含量的基因的遗传标记。根据方差分析（ANOVA），一个RAPD标记（25a）与亚麻酸含量显著相关；具有该标记的F2个体（包括纯合子和杂合子）种子中的亚麻酸浓度为7.43±1.35%，而缺乏该标记的个体种子中的亚麻酸浓度为5.70±1.52%。标记25a可用于在未来的油菜育种计划中促进脂肪酸组成的选择。
