通过反相高效液相色谱法合成和分离保护的三肽差向异构体
Synthesis and separation of protected tripeptide epimers by RP-HPLC.
作者信息
Griehl C, Merkel S
机构信息
Department of Organic Chemistry, Martin Luther University Halle-Wittenberg, Germany.
出版信息
Int J Pept Protein Res. 1995 Mar;45(3):217-24. doi: 10.1111/j.1399-3011.1995.tb01483.x.
Abstract
Twenty three epimeric pairs of protected tripeptides of the type Z-Ala-Xaa-Val-OMe have been synthesized by conventional coupling procedures in solution. Optimal conditions for baseline separation of LLL/LDL epimers were found by high-performance liquid chromatography on a RP-18 column with aqueous methanol as mobile phase. The hydrophobic parameters (log kw) were determined for all tripeptides.
摘要
通过溶液中的常规偶联方法合成了23对Z-Ala-Xaa-Val-OMe型的受保护三肽差向异构体对。在以甲醇水溶液为流动相的RP-18柱上，通过高效液相色谱法找到了LLL/LDL差向异构体基线分离的最佳条件。测定了所有三肽的疏水参数（log kw）。
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