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通过聚合酶链反应和SH基因的核苷酸测序鉴别疫苗株和野生型腮腺炎病毒:简要报告

Differentiation of vaccine and wild mumps viruses by polymerase chain reaction and nucleotide sequencing of the SH gene: brief report.

作者信息

Künkel U, Driesel G, Henning U, Gerike E, Willers H, Schreier E

机构信息

Robert Koch Institut des Bundesgesundheitsamtes, Berlin, Germany.

出版信息

J Med Virol. 1995 Feb;45(2):121-6. doi: 10.1002/jmv.1890450202.

DOI:10.1002/jmv.1890450202
PMID:7775928
Abstract

The nucleotide sequences of the SH gene and its F gene flanking region were determined over a range of 322 nucleotides for two live vaccines, two vaccine-associated isolates, six wild mumps viruses, and an attenuated mumps virus and compared to other sequences already published. Comparison revealed that the vaccine strains were clearly different from each other and the postvaccination isolates were different from the vaccines used. The viruses were assigned to three known cocirculating viral lineages. The attenuated mumps virus possesses nucleotide sequences identical to those of its progenitor strain.

摘要

测定了两种活疫苗、两种疫苗相关分离株、六种野生腮腺炎病毒和一种减毒腮腺炎病毒的SH基因及其F基因侧翼区域322个核苷酸范围的核苷酸序列,并与已发表的其他序列进行了比较。比较结果显示,疫苗株彼此明显不同,接种疫苗后的分离株与所用疫苗也不同。这些病毒被分为三个已知的共同流行病毒谱系。该减毒腮腺炎病毒的核苷酸序列与其亲本株相同。

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引用本文的文献

1
Mumps: an Update on Outbreaks, Vaccine Efficacy, and Genomic Diversity.流行性腮腺炎:暴发、疫苗效力和基因组多样性的最新研究进展
Clin Microbiol Rev. 2020 Feb 26;33(2). doi: 10.1128/CMR.00151-19. Print 2020 Mar 18.
2
A new mumps virus lineage found in the 1995 mumps outbreak in western Switzerland identified by nucleotide sequence analysis of the SH gene.通过对SH基因进行核苷酸序列分析,在瑞士西部1995年腮腺炎疫情中发现了一种新的腮腺炎病毒谱系。
Arch Virol. 1996;141(3-4):733-41. doi: 10.1007/BF01718331.
3
Molecular characterization of mumps virus strains circulating during an epidemic in eastern Switzerland 1992/93.
1992/93年瑞士东部流行性腮腺炎流行期间流行的腮腺炎病毒株的分子特征
Arch Virol. 1994;136(3-4):433-8. doi: 10.1007/BF01321072.