Adi M M, Chisholm D M, Waterhouse J P
Department of Dental Surgery and Periodontology, University of Dundee, Scotland.
J Oral Pathol Med. 1995 Mar;24(3):130-5. doi: 10.1111/j.1600-0714.1995.tb01153.x.
The emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. An avidin-biotin technique was used to study the binding of lectins from Ulex europeus I (UEA-I), Dolichos biflorus (DBA), Glycine maximus (SBA), Helix pomatia (HPA), Arachis hypogaea (PNA) and Triticum vulgare (WGA) to specific sugars on sections of tissue from labial glands, glands of Blandin and Nuhn, glands of von Ebner and the dorsoposterior lingual salivary glands. Incipient synthesis of glycoconjugates in early glands and their presence in the cells and ducts of the later glands was shown. The study also showed a time-related increase in both staining intensity and binding sites of serous acinar cells from all glands and for all lectins used. For mucous cells, peak intensity of staining was reached by the middle phase of development. During later gland development this intensity was maintained in dorsoposterior lingual glands but tended to decline in labial glands. The various lectins showed different degrees of binding but UEA-I lectin generally bound the L-fucose sugar group in all salivary glands at all gestational ages. The results showed that lectins appear to bind to the oligosaccharides on epithelial cell surfaces of fetal salivary glands at all stages of development. The degree of change depends upon the stage of differentiation and maturation of the glands.
通过凝集素组织化学研究了发育中的人类胎儿唇腺和舌腺中含有特定寡糖的糖缀合物的新合成情况。采用抗生物素蛋白-生物素技术研究了欧洲荆豆凝集素（UEA-I）、双花扁豆凝集素（DBA）、大豆凝集素（SBA）、苹果蜗牛凝集素（HPA）、花生凝集素（PNA）和普通小麦凝集素（WGA）与唇腺、布兰丁和努恩腺、埃布纳腺以及舌背后部唾液腺组织切片上特定糖类的结合情况。结果显示了早期腺体中糖缀合物的初步合成以及后期腺体细胞和导管中糖缀合物的存在。该研究还表明，所有腺体的浆液性腺泡细胞以及所用的所有凝集素，其染色强度和结合位点均呈现与时间相关的增加。对于黏液细胞，在发育中期达到染色强度峰值。在腺体发育后期，舌背后部腺体中这种强度得以维持，但唇腺中的强度则趋于下降。各种凝集素显示出不同程度的结合，但UEA-I凝集素通常在所有孕周的所有唾液腺中都能结合L-岩藻糖糖基。结果表明，凝集素似乎在发育的各个阶段都能与胎儿唾液腺上皮细胞表面的寡糖结合。变化程度取决于腺体的分化和成熟阶段。
