Pedini V, Gargiulo A M, Ceccarelli P
Istituto di Anatomia degli Animali Domestici, Università degli Studi di Perugia, Italy.
Anat Histol Embryol. 1997 Sep;26(3):223-6. doi: 10.1111/j.1439-0264.1997.tb00130.x.
The Bovine tongue possesses numerous circumvallate papillae (8-16 each side). The troughs around the papillae are the openings of the ducts of the gustatory (von Ebner's) glands. In this study, we have characterized in situ the glycosidic composition of the secretion of bovine gustatory glands using traditional histochemical methods and lectin histochemistry with and without prior neuraminidase (sialidase) digestion. The lectin-horseradish peroxidase conjugates employed were: PNA, DBA, SBA, WGA, LTA, UEA I and ConA. Acinar cells show a diffuse positivity towards PAS and Alcian blue at pH 2.5 and the most intense and homogeneous lectin staining was obtained with PNA. This indicates that bovine gustatory glands secrete glycoproteins with 1,2-glycol containing hexoses and carboxyl-rich glycoconjugates and that galactosyl (beta 1-->3) Nacetylgalactosamine is the most frequent sugar residue present in these glycoproteins. Results were compared with data reported in the literature on the same glands of other species.
牛舌有许多轮廓乳头（每侧8 - 16个）。乳头周围的沟是味觉（冯·埃布纳氏）腺导管的开口。在本研究中，我们使用传统组织化学方法以及在有无事先神经氨酸酶（唾液酸酶）消化的情况下进行凝集素组织化学，对牛味觉腺分泌物的糖苷组成进行了原位表征。所使用的凝集素 - 辣根过氧化物酶结合物有：PNA、DBA、SBA、WGA、LTA、UEA I和ConA。腺泡细胞在pH 2.5时对PAS和阿尔辛蓝呈弥漫性阳性反应，用PNA获得的凝集素染色最强烈且均匀。这表明牛味觉腺分泌含有含1,2 - 二醇己糖和富含羧基的糖缀合物的糖蛋白，并且半乳糖基（β1→3）N - 乙酰半乳糖胺是这些糖蛋白中最常见的糖残基。将结果与文献中报道的其他物种相同腺体的数据进行了比较。
