Brundege J M, Dunwiddie T V
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
Neurosci Lett. 1995 Feb 17;186(2-3):214-8. doi: 10.1016/0304-3940(95)11320-v.
Acetate is the primary product of ethanol catabolism and can accumulate in the blood at concentrations of up to 2 mM following ethanol consumption. It has been suggested that some of the pharmacological actions of ethanol are mediated via acetate, which can lead indirectly to the release of endogenous adenosine. In the present experiments this hypothesis was tested by examining the effects of exogenous sodium acetate on the physiology of hippocampal slices from rat brain. Acetate had no significant effect on intracellular responses recorded from CA1 pyramidal neurons or on extracellular field potentials evoked from the either the CA1 region or the dentate gyrus. There was also no significant difference in responses to the adenosine receptor antagonist theophylline in CA1 pyramidal neurons recorded using intracellular filling solutions containing potassium acetate, KCl, or potassium methylsulfate. These results suggest that the presence of acetate, either in the extracellular medium or within an intracellular electrode, does not induce a significant increase in adenosine receptor activation in the hippocampus.
乙酸盐是乙醇分解代谢的主要产物，饮酒后其在血液中的浓度可累积至高达2 mM。有人提出，乙醇的一些药理作用是通过乙酸盐介导的，乙酸盐可间接导致内源性腺苷的释放。在本实验中，通过检测外源性乙酸钠对大鼠脑海马切片生理学的影响来验证这一假说。乙酸盐对CA1锥体神经元记录的细胞内反应或CA1区域或齿状回诱发的细胞外场电位均无显著影响。在使用含有乙酸钾、氯化钾或甲磺酸钾的细胞内灌流液记录的CA1锥体神经元中，对腺苷受体拮抗剂茶碱的反应也没有显著差异。这些结果表明，无论是细胞外培养基中还是细胞内电极内存在乙酸盐，都不会导致海马体中腺苷受体激活显著增加。
