Aladysheva Zh I, Kaminskaia G O
Probl Tuberk. 1995(2):20-3.
PAF levels in native blood neutrophils before and after 1-hour incubation with killed BCG culture and in relevant incubation medium were measured in 44 patients with active pulmonary tuberculosis according to rabbit platelet aggregation. A control group consisted of 12 healthy volunteers. Neutrophil PAF concentrations in the patients were 6 times higher than those in the controls and caused a rise in plasma PAF levels. Intracellular PAF content was related to the process characteristics (new-onset tuberculosis, exacerbation, recurrence), intoxication, hemogram shifts and immune status of the patient. In vitro experiments showed that in response to a specific phagocytosis object, generation of PAF in neutrophils and its release into the intercellular space depend on initial PAF levels in native cells. In normal and moderately elevated PAF in native neutrophils contact with killed BCG culture entailed its intracellular content increase, while in initially high intracellular PAF additional stimulation induced primarily its release.
根据兔血小板聚集情况，对44例活动性肺结核患者在与灭活卡介苗培养物孵育1小时前后以及在相关孵育培养基中的天然血液中性粒细胞中的PAF水平进行了测量。对照组由12名健康志愿者组成。患者中性粒细胞中的PAF浓度比对照组高6倍，并导致血浆PAF水平升高。细胞内PAF含量与患者的病情特征（新发肺结核、病情加重、复发）、中毒、血常规变化和免疫状态有关。体外实验表明，响应特定吞噬对象时，中性粒细胞中PAF的生成及其释放到细胞间空间取决于天然细胞中的初始PAF水平。在天然中性粒细胞中PAF正常和适度升高的情况下，与灭活卡介苗培养物接触会使其细胞内含量增加，而在最初细胞内PAF较高时，额外刺激主要诱导其释放。
