Kaminskaia G O, Aladysheva Zh I
Vestn Ross Akad Med Nauk. 1995(7):45-8.
The content of platelet-activating factor (PAF) in plasma, blood, native neutrophils, in the same neutrophils after their incubation with a killed BGC culture and in the incubation medium was measured in 74 patients with various types of active pulmonary tuberculosis. Twelve healthy volunteers formed a control group. PAF was measured by the rabbit platelet aggregation technique. Higher plasma PAF levels were found in half the patients. The highest plasma PAF concentration was observed in the newly revealed patients, in cases with forming cavities in the lung tissue, in those discharged a lot of sputum Mycobacterium tuberculosis, and in those with lymphopenia. PAF content in the native neutrophils of patients was 10 times as high as that in healthy persons. Incubation with the killed BCG culture had no influence on PAF content in the control neutrophils, but greatly increased individual differences in patients. The concentration of PAF in the incubation media of stimulated neutrophils from tuberculosis patients was 3 times higher than in the controls.
对74例不同类型的活动性肺结核患者，测定了其血浆、血液、天然中性粒细胞、用灭活的卡介苗培养物孵育后的同一中性粒细胞以及孵育培养基中血小板活化因子（PAF）的含量。12名健康志愿者组成对照组。采用兔血小板聚集技术测定PAF。半数患者血浆PAF水平较高。在新发现的患者、肺组织有空洞形成的病例、痰中排出大量结核分枝杆菌的患者以及淋巴细胞减少的患者中，观察到血浆PAF浓度最高。患者天然中性粒细胞中的PAF含量是健康人的10倍。用灭活的卡介苗培养物孵育对对照中性粒细胞中的PAF含量没有影响，但大大增加了患者个体间的差异。结核病患者受刺激的中性粒细胞孵育培养基中PAF的浓度比对照组高3倍。
