尼泊尔两个不同地理区域弓形虫病的血清流行病学研究。

Seroepidemiological study of toxoplasmosis in two different geographical areas in Nepal.

作者信息

Rai S K, Shibata H, Sumi K, Kubota K, Hirai K, Matsuoka A, Kubo T, Tamura T, Basnet S R, Shrestha H G

机构信息

Department of Clinical Laboratory, Hyogo College of Medicine Hospital, Japan.

出版信息

Southeast Asian J Trop Med Public Health. 1994 Sep;25(3):479-84.

PMID:7777911

Abstract

A total of 302 serum samples collected from Chitawan (159) and Mustang (143) districts of Nepal were included in this study. Anti-toxoplasma antibody was detected using micro-latex agglutination (MLA) and ELISA methods. An overall positive rate was found to be 57.9%. The positive rate in Chitawan was significantly higher (64.1%) (less than 1,000 m altitude) compared to that in Mustang (51.0%) (more than 3,000 m altitude) (p < 0.05). Females in Chitawan showed significantly higher positive rate (71.2%) compared to males (56.9%) (p < 0.05). On the contrary, though insignificantly, males showed higher positive rate (57.9%) compared to that of females (43.3%) in Mustang. Almost equal positive rate was observed among males in both study area. Females in Chitawan showed significantly higher (71.2%) positive rate compared to their counterparts in Mustang (43.3%) (p < 0.001). A slight increase in positive rate with age was observed in Chitawan while in Mustang a decreasing trend was noticed. Ethnically though statistically not significant, Indo-Aryans showed a higher positive rate (69.2%) compared to the positive rate shown by Tibeto-Burmans (63.1%) in Chitawan while the reverse was true in Mustang (Tibeto-Burmans: 53.8% and Indo-Aryans: 38.4%). Interestingly, 2.9% and 1.3% of MLA positive samples showed toxoplasma IgM antibody. None of the IgM positive samples were positive for toxoplasmic antigens.

摘要

本研究纳入了从尼泊尔奇旺（159份）和木斯塘（143份）地区采集的共302份血清样本。采用微乳胶凝集试验（MLA）和酶联免疫吸附测定（ELISA）方法检测抗弓形虫抗体。总体阳性率为57.9%。奇旺地区（海拔低于1000米）的阳性率（64.1%）显著高于木斯塘地区（海拔高于3000米）（51.0%）（p<0.05）。奇旺地区女性的阳性率（71.2%）显著高于男性（56.9%）（p<0.05）。相反，在木斯塘地区，男性的阳性率（57.9%）虽不显著高于女性（43.3%）。两个研究地区男性的阳性率几乎相等。奇旺地区女性的阳性率（71.2%）显著高于木斯塘地区的女性（43.3%）（p<0.001）。在奇旺地区，阳性率随年龄略有上升，而在木斯塘地区则呈下降趋势。在奇旺地区，印度-雅利安人的阳性率（69.2%）高于藏缅族（63.1%），尽管在统计学上不显著；而在木斯塘地区情况相反（藏缅族：53.8%，印度-雅利安人：38.4%）。有趣的是，2.9%的MLA阳性样本和1.3%的ELISA阳性样本显示有弓形虫IgM抗体。所有IgM阳性样本的弓形虫抗原均为阴性。