Okunieff P, Abraham E H, Moini M, Snyder M L, Gloe T R, Capogrossi M C, Ding I
Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Acta Oncol. 1995;34(3):435-8. doi: 10.3109/02841869509094004.
We examined the potential of bFGF to function as a radioprotector of bone marrow (BM). Total intravenous doses of bFGF ranged from 1 to 24 micrograms/mouse, in 2 divided doses. Whole body radiation (WBI) was given in a single fraction to C3H mice. Histologic observations were performed on femur BM at various times after bFGF (or placebo) treatment. Thigh radiation in thigh-tumor bearing mice was delivered in a single fraction. bFGF increased the LD50/30 of mice in a dose dependent fashion, with an apparent maximum protection obtained with > or = 6 micrograms given half 24 h and half 4 h before irradiation. BM histology shows prominent recovery of megakaryocytes and all cell lineages along with less loss in cellularity compared to control irradiated animals. No radioprotection of RIF1 tumors after bFGF was detected. These results indicate that bFGF may be a selective radioprotector of normal tissue.
我们研究了碱性成纤维细胞生长因子（bFGF）作为骨髓（BM）辐射防护剂的潜力。bFGF的静脉注射总剂量为1至24微克/小鼠，分两次给药。对C3H小鼠进行单次全身照射（WBI）。在bFGF（或安慰剂）治疗后的不同时间对股骨骨髓进行组织学观察。对荷瘤小鼠的大腿进行单次局部照射。bFGF以剂量依赖性方式提高了小鼠的半数致死剂量（LD50/30），在照射前24小时和4小时各给予≥6微克时获得明显的最大保护作用。骨髓组织学显示，与对照照射动物相比，巨核细胞和所有细胞谱系显著恢复，细胞数量损失较少。未检测到bFGF对RIF1肿瘤的辐射防护作用。这些结果表明，bFGF可能是正常组织的选择性辐射防护剂。
