Pandurangi R S, Kuntz R R, Volkert W A
Department of Chemistry, University of Missouri, Columbia, USA.
Appl Radiat Isot. 1995 Apr;46(4):233-9. doi: 10.1016/0969-8043(94)00139-q.
N-alkyl derivatives of perfluoroarylazides are excellent candidates for photolabeling of proteins since they have absorption spectra in the 340-350 nm range permitting photolabel absorption without direct protein photolysis. The [14C]-N-methylamino derivative of 4-azido-tetrafluorobenzonitrile has been used to demonstrate that 80% of the photo-induced nitrene transient becomes covalently attached to HSA during photolysis. Multiwavelength detection of the photoprobe-protein separation by size exclusion chromatography is shown to be an effective tool for assessing the conjugation of the photoprobe to the protein.
全氟芳基叠氮化物的N-烷基衍生物是蛋白质光标记的理想选择，因为它们在340-350nm范围内有吸收光谱，可实现光标记吸收而不会直接导致蛋白质光解。4-叠氮基-四氟苯腈的[14C]-N-甲基氨基衍生物已被用于证明在光解过程中，80%的光诱导氮烯瞬态会与HSA共价结合。通过尺寸排阻色谱对光探针-蛋白质分离进行多波长检测，是评估光探针与蛋白质结合情况的有效工具。
