用[¹⁴C]4-叠氮基-2,3,5,6-四氟苯甲酸甲酯对人血清白蛋白和人γ-球蛋白进行高效光标记
High efficiency photolabeling of human serum albumin and human gamma-globulin with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate.
作者信息
Pandurangi R S, Karra S R, Kuntz R R, Volkert W A
机构信息
Department of Chemistry, H.S. Truman Memorial VA Hospital, University of Missouri, Columbia 65211, USA.
出版信息
Bioconjug Chem. 1995 Sep-Oct;6(5):630-4. doi: 10.1021/bc00035a019.
The efficiency of photolabeling of HSA and IgG with [14C]methyl 4-azido-2,3,5,6-tetrafluorobenzoate has been studied using size exclusion chromatography in conjunction with liquid scintillation counting. Labeling efficiencies of 78% for HSA and 82% for IgG have been determined. The extent of bond insertion into proteins exceeds the C-H insertion efficiency in cyclohexane with less wastage into anilinium and azo side products. These results suggest that the photoprobe accesses hydrophobic regions of both proteins prior to photolysis.
使用尺寸排阻色谱结合液体闪烁计数法，研究了用[¹⁴C]甲基4-叠氮基-2,3,5,6-四氟苯甲酸酯对人血清白蛋白（HSA）和免疫球蛋白G（IgG）进行光标记的效率。已确定HSA的标记效率为78%，IgG的标记效率为82%。与环己烷中的C-H插入效率相比，键插入蛋白质的程度更高，转化为苯胺鎓盐和偶氮副产物的损耗更少。这些结果表明，光探针在光解之前进入了两种蛋白质的疏水区域。
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