Colquhoun I J, Defernez M, Morris V J
Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, United Kingdom.
Carbohydr Res. 1995 Apr 19;269(2):319-31. doi: 10.1016/0008-6215(94)00367-o.
Acetan is a bacterial polysaccharide produced by Acetobacter xylinum NRRL B42. Chemical mutagenesis of A.xylinum allowed selection of a mutant strain which produced a new polysaccharide, CR1/4. 2D NMR methods have been used to assign the 1H and 13C spectra of the two polysaccharides and to determine that CR1/4 has the structure shown below. The total number of O-acetyl groups is slightly less than two per repeating unit. [formula: see text] The pentasaccharide side chain of acetan is truncated to a disaccharide unit in CR1/4, but the structures are otherwise identical. In particular, the degree of acetylation is about the same and the O-acetyl groups are located at the same position in both polysaccharides.
醋杆菌聚糖是由木醋杆菌NRRL B42产生的一种细菌多糖。对木醋杆菌进行化学诱变可筛选出一种能产生新多糖CR1/4的突变菌株。二维核磁共振方法已用于确定这两种多糖的1H和13C谱，并确定CR1/4具有如下所示的结构。每个重复单元的O - 乙酰基总数略少于两个。[化学式：见原文]醋杆菌聚糖的五糖侧链在CR1/4中截短为二糖单元，但其他结构相同。特别是，两种多糖的乙酰化程度大致相同，且O - 乙酰基位于相同位置。
