MacCormick C A, Harris J E, Gunning A P, Morris V J
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, UK.
J Appl Bacteriol. 1993 Feb;74(2):196-9. doi: 10.1111/j.1365-2672.1993.tb03015.x.
Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.
木醋杆菌NRRL B42（NCIB 40123）可产生纤维素和一种名为乙酰聚糖的复杂阴离子支链杂多糖。利用化学诱变来分离稳定的纤维素缺失型木醋杆菌突变体。对这些纤维素缺失型木醋杆菌进一步进行化学诱变，以筛选出分泌乙酰聚糖结构变体多糖的突变体。本文描述了这些多糖的制备、纯化及特性。对多糖结构CR1/4的甲基化分析表明，该多糖具有乙酰聚糖结构，其侧链截短，末端为葡萄糖醛酸。
