PTF1在酿酒酵母中编码一种必需蛋白，该蛋白与一个新的假定肽脯氨酰顺反异构酶家族具有很强的同源性。
PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases.
作者信息
Hani J, Stumpf G, Domdey H
机构信息
Genzentrum, Ludwig-Maximilians-Universität München, Germany.
出版信息
FEBS Lett. 1995 May 29;365(2-3):198-202. doi: 10.1016/0014-5793(95)00471-k.
Complementation of a temperature sensitive mutant of the yeast Saccharomyces cerevisiae resulted in the isolation of PTF1 (processing/termination factor 1), an essential gene encoding a putative 3'-end processing or transcription termination factor of pre-mRNAs. Ptf1p shows significant homology to a newly discovered family of PPIases. This family is characterized by its insensitivity to immunosuppressive drugs and the lack of homology with cyclophilins and FK-506 binding proteins [Rahfeld et al. (1994) FEBS Lett. 352, 180-184]. Should Ptf1p display PPIase activity, it would be the first characterized, eukaryotic member of this putative family, which is essential for growth.
对酿酒酵母温度敏感突变体进行互补作用，从而分离出了PTF1（加工/终止因子1），这是一个必需基因，编码一种假定的前体mRNA 3'端加工或转录终止因子。Ptf1p与新发现的一类肽基脯氨酰顺反异构酶（PPIase）具有显著的同源性。该家族的特点是对免疫抑制药物不敏感，且与亲环蛋白和FK-506结合蛋白缺乏同源性[拉费尔德等人（1994年），《欧洲生物化学学会联合会快报》352卷，第180 - 184页]。如果Ptf1p具有PPIase活性，它将是这个假定家族中首个被鉴定的对生长至关重要的真核成员。
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