Macleod I C, Irvine D S
Department of Obstetrics and Gynaecology, University of Aberdeen, Aberdeen Maternity Hospital, Foresterhill, UK.
Hum Reprod. 1995 Mar;10(3):580-6. doi: 10.1093/oxfordjournals.humrep.a135993.
In this paper we examine the value of both conventional and computer-assisted semen analysis (CASA) using the Hamilton-Thorn HTM-S 2030 in predicting the in-vivo fertility of cryopreserved donor semen. Semen samples were examined prospectively and data on the conventional criteria of semen quality, sperm morphometry and movement were collected. Of 61 ejaculates identified, 33 achieved pregnancies ('successful') and 28 failed to do so ('unsuccessful'), despite insemination into at least four different normal female recipients. When the post-thaw semen profiles were compared, no differences were observed between the two groups in respect of the conventional criteria of semen quality determined by conventional laboratory techniques; however, there were differences in respect of both morphometry and movement characteristics determined by the HTM-S. When multiple logistic regression was used to examine the ability of the variables measured to predict the achievement of pregnancy, the conventional criteria of semen quality were of no value (chi 2 = 6.67, P = 0.353). However, the CASA assessment successfully predicted outcome in 86.9% of cases (chi 2 = 44.3, P = 0.0021). It was concluded that CASA assessment is of significant value in predicting the ability of an ejaculate to achieve pregnancy.
在本文中，我们使用汉密尔顿-桑恩HTM-S 2030检测传统精液分析和计算机辅助精液分析（CASA）在预测冷冻保存的供体精液体内受精能力方面的价值。对精液样本进行前瞻性检测，并收集有关精液质量、精子形态学和运动的传统标准的数据。在鉴定出的61份射精样本中，33份使女性受孕（“成功”），28份未受孕（“失败”），尽管已将精液至少授精给了四名不同的正常女性受体。比较解冻后的精液特征时，在通过传统实验室技术确定的精液质量传统标准方面，两组之间未观察到差异；然而，在通过HTM-S测定的形态学和运动特征方面存在差异。当使用多元逻辑回归分析来检测所测变量预测受孕成功率的能力时，精液质量的传统标准没有价值（卡方=6.67，P=0.353）。然而，CASA评估在86.9%的病例中成功预测了结果（卡方=44.3，P=0.0021）。得出的结论是，CASA评估在预测射精样本的受孕能力方面具有重要价值。
