Martel-Renoir D, Grunewald V, Touitou R, Schwaab G, Joab I
CNRS URA 1301, Institut Gustave Roussy, Villejuif, France.
J Gen Virol. 1995 Jun;76 ( Pt 6):1401-8. doi: 10.1099/0022-1317-76-6-1401.
We recently showed that BZLF1, the gene encoding the Epstein-Barr virus (EBV) ZEBRA protein, was expressed in all eight nasopharyngeal carcinoma (NPC) specimens studied. We present here studies on the expression of EBV lytic cycle genes in the same eight NPC biopsies to determine if production of the ZEBRA transactivator could lead to a complete productive cycle. The tumour lesions exhibit a number of different patterns of limited lytic gene expression. In three out of eight tumours neither BRLF1 nor BMLF1 expression could be detected. Otherwise BMLF1 mRNA was expressed in all the other specimens. Three specimens also expressed BRLF1. Two specimens not only exhibited BZLF1, BMLF1 and BRLF1 transcripts, but also expressed the late gene BLLF1 which encodes the membrane protein gp220. The early gene product BBLF2 could not be detected in any of the eight NPC. However, expression of the late gene encoding the lytic truncated form of LMP1 (D1LMP) was found in seven of the eight NPC biopsies. Thus, it could be suggested that the EBV abortive lytic cycle occurred in most of the NPC studied.
我们最近发现，编码爱泼斯坦-巴尔病毒（EBV）ZEBRA蛋白的基因BZLF1在所研究的全部8份鼻咽癌（NPC）标本中均有表达。在此，我们展示了对同一8份NPC活检标本中EBV裂解周期基因表达的研究，以确定ZEBRA反式激活因子的产生是否会导致完整的增殖周期。肿瘤病变呈现出多种不同的有限裂解基因表达模式。在8个肿瘤中的3个中，未检测到BRLF1和BMLF1的表达。否则，BMLF1 mRNA在所有其他标本中均有表达。3个标本也表达BRLF1。2个标本不仅呈现出BZLF1、BMLF1和BRLF1转录本，还表达了编码膜蛋白gp220的晚期基因BLLF1。在8份NPC标本中的任何一份中均未检测到早期基因产物BBLF2。然而，在8份NPC活检标本中的7份中发现了编码LMP1裂解截短形式（D1LMP）的晚期基因的表达。因此，可以推测在所研究的大多数NPC中发生了EBV流产性裂解周期。
