Fernández-Gutiérrez B, Hernández-García C, Bañares A A, Jover J A
Service of Rheumatology, Hospital Universitario San Carlos, Madrid, Spain.
J Rheumatol. 1995 Mar;22(3):413-20.
To study CD69+ synovial fluid (SF) T cells and the mechanisms regulating CD69 expression in rheumatoid arthritis (RA).
One or 2 color flow cytometry was used to determine CD69 and other surface markers. Cultures of SF T cells alone or mixed with autologous SF non-T cells were used for CD69 maintenance assays.
SF T cells were enriched in CD69+. These cells were mainly CD3+, CD8+ and CD25-. CD69 was maintained on SF T cells cultured with SF non-T cells but not when the former were cultured alone or in the presence of different supernatants from RA SF T and non-T cells cultures with sustained CD69 expression. Pretreatment of T and non-T cells with anti-CD18 monoclonal antibody inhibited CD69 expression, while paraformaldehyde-"fixed" non-T cells effectively maintained it.
SF T cells exhibit a phenotype with evidence of past and recent activation. Our studies demonstrate that most of the recently activated SF T cells are CD8+. We also found that continuous cell-to-cell interaction between T and non-T cells are responsible for the maintenance of this particular state of activation of SF T cells.
研究类风湿关节炎（RA）中CD69 + 滑膜液（SF）T细胞及调节CD69表达的机制。
采用单或双色流式细胞术检测CD69及其他表面标志物。单独培养SF T细胞或与自体SF非T细胞混合培养用于CD69维持试验。
SF T细胞富含CD69 + 。这些细胞主要为CD3 + 、CD8 + 和CD25 - 。与SF非T细胞共培养时，SF T细胞上的CD69得以维持，但单独培养或在来自持续表达CD69的RA SF T细胞和非T细胞培养物的不同上清液存在下培养时则不然。用抗CD18单克隆抗体预处理T细胞和非T细胞可抑制CD69表达，而多聚甲醛“固定”的非T细胞可有效维持其表达。
SF T细胞表现出具有既往和近期活化证据的表型。我们的研究表明，大多数近期活化的SF T细胞为CD8 + 。我们还发现，T细胞与非T细胞之间持续的细胞间相互作用负责维持SF T细胞的这种特定活化状态。
