Liu Y S, Du W P, Wu Y M, Chen Y G, Zheng K Y, Shi J M, Hu X Z, Li G Y, You C F, Wu Z X
Department of Parasitology, Xuzhou Medical College, Jiangsu, China.
J Trop Med Hyg. 1995 Jun;98(3):151-4.
All 35 confirmed clonorchiasis cases showed a positive reaction in dot-immunogold-silver staining (Dot-IGSS) with a mean serum titre of 1:1656, while none of the sera from 35 normal individuals reacted. A seroepidemiological survey of middle-school students revealed a positive rate of 17.0% (142/836), and 76.1% (105/138) of the serologically positive students were egg positive in stool examination. The egg positive rates in those with antibody at levels of 1+ to 4+ were 57.1% (28/49), 75.7% (28/37), 94.7% (36/38) and 92.9% (13/14) respectively. It is believed that Dot-IGSS can be used for the clinical diagnosis and epidemiological survey of clonorchiasis.
35例确诊华支睾吸虫病病例在斑点免疫金银染色（Dot-IGSS）中均呈阳性反应，血清平均滴度为1:1656，而35名正常个体的血清均无反应。一项对中学生的血清流行病学调查显示，阳性率为17.0%（142/836），血清学阳性学生中76.1%（105/138）粪便检查虫卵阳性。抗体水平为1+至4+者的虫卵阳性率分别为57.1%（28/49）、75.7%（28/37）、94.7%（36/38）和92.9%（13/14）。认为Dot-IGSS可用于华支睾吸虫病的临床诊断和流行病学调查。
