Liu Y, Zheng K, Chen M, Fu L, Du W, Shi Z
Department of Parasitology, Xuzhou Medical College, Jiangsu, PR China.
Southeast Asian J Trop Med Public Health. 2001 Sep;32(3):558-61.
To prevent post-transfusion toxoplasmosis, the sera of blood donors, per six of which were mixed as a pool, were examined for anti-Toxoplasma gondii antibiodies by Dot-immunogold silver staining (Dot-IGSS) with the single serum specimens examined simultaneously. The results showed that the sensitivity and specificity of serum pool method were 92.31% and 99.96% respectively. The consistent rate between the two methods was 99.73% and kappa value was 0.947 (p<0.01). Considering the mean infection rate of Toxoplasma gondii being 4.86% in China, if the serum pool method be adoped, with pool size k=5, a 57% reduction in the number of tests, as well as the cost of the screen, can be expected. Beside the social benefit, consequent upon the interruption of the Toxoplasma gondii infection spread through blood transfusion also can be expected.
为预防输血后弓形虫病，将献血者血清每6份混合为1池，采用斑点免疫金银染色法（Dot-IGSS）检测抗弓形虫抗体，同时对单个血清标本进行检测。结果显示，血清混合法的灵敏度和特异度分别为92.31%和99.96%。两种方法的符合率为99.73%，kappa值为0.947（p<0.01）。考虑到我国弓形虫平均感染率为4.86%，若采用血清混合法，混合样本量k = 5时，检测次数及筛查成本预计可降低57%。除社会效益外，还可预期因输血传播弓形虫感染中断而带来的效益。
