Nakata N, Tozawa T
Department of Clinical Laboratories, Hyogo College of Medicine, Nishinomiya.
Rinsho Byori. 1995 May;43(5):508-12.
Reference intervals of the activity of serum alkaline phosphatase isozymes (AP-IZ: high molecular-, liver-, bone- and intestinal-types) were determined according to the ABO (blood type) system in 200 healthy subjects aged 20-39 years. AP activity was determined according to the JSCC method. AP-IZ was stained by the formazan method after isolation by TITANIII-Lipo plate electrophoresis. For the electrophoresis, treated serum with neuraminidase and untreated one were concomitantly used for detecting liver AP and bone AP respectively. As a result of comparison of mean AP-IZ activity among the groups divided according to the ABO system, total AP, intestinal AP and liver AP activities in the type B and O persons were significantly higher than in the type A and AB persons. It is well known that the activities of total AP and intestinal AP in type B and O persons are higher than in type A and AB persons, but there have been no reports showing that the activity of liver AP in type B and O persons is higher than in type A and AB persons. Furthermore, in the type B and O persons there was a low correlation (r = 0.195, p < 0.05) between the activities of liver AP and intestinal AP. The present assessment included subjects in the age group (20-39 years) considered not to show fluctuations in the activity of bone AP, which is influenced by age. The above findings should be investigated in regard to other age groups.
在200名20 - 39岁的健康受试者中，根据ABO血型系统测定了血清碱性磷酸酶同工酶（AP - IZ：高分子型、肝型、骨型和肠型）的活性参考区间。AP活性根据日本临床化学学会（JSCC）方法测定。通过TITANIII - Lipo平板电泳分离后，采用甲臜法对AP - IZ进行染色。对于电泳，分别使用经神经氨酸酶处理的血清和未经处理的血清来检测肝型AP和骨型AP。根据ABO系统分组比较各组平均AP - IZ活性的结果显示，B型和O型人的总AP、肠型AP和肝型AP活性显著高于A型和AB型人。众所周知，B型和O型人的总AP和肠型AP活性高于A型和AB型人，但尚无报道表明B型和O型人的肝型AP活性高于A型和AB型人。此外，在B型和O型人中，肝型AP和肠型AP活性之间的相关性较低（r = 0.195，p < 0.05）。本评估纳入的年龄组（20 - 39岁）被认为骨型AP活性不受年龄影响而无波动。上述发现应在其他年龄组中进行研究。
