Sasaki M, Shimizu K, Fukushima T, Shiono H
Department of Legal Medicine, Asahikawa Medical College, Hokkaido, Japan.
Nihon Hoigaku Zasshi. 1995 Apr;49(2):70-9.
We attempted sex and individual identifications of several forensic specimens by detecting various sex chromosome-specific sequences by the polymerase chain reaction (PCR) method. The specimens were 30 blood stains that were attached to gauze and stored for 11 months -15 years (15 males, 14 females and 1 of unknown gender) and 10 bleached white bones (8- about 10000 years after death). Of the known 29 blood stains, the sex determination rate was the highest by DYZ-1 (amplified DNA fragment = 149bp, about 3000 copies on Y chromosome) recombinated with DXZ-1 (28 of the 29 samples) although 27 samples could be determined by DYZ-1 (amplified DNA fragment = 1000bp, about 3000 copies on Y chromosome) recombinated with DXZ-1. The sex determination rate was relatively low by DYZ-3 (amplified DNA fragment = 170bp, about 100 copies on Y chromosome) recombinated with DXZ-1 (23 of the 29 samples). The sex determination rate by two single locus markers, PAB or Amelogenin was markedly low (7 and 9 of 29 samples, respectively). The sex determination rate by the 27H39 locus was 27 of the 29 samples; 14 of the 15 male were determined (A = 186bp, 1; B = 190bp, 7; C = 194bp, 5; D = 198bp, 1; E = 202bp, 0). One unknown gender could be identified as male by multiple locus markers, but could not be identified by any single locus markers except for the 27H39 locus.(ABSTRACT TRUNCATED AT 250 WORDS)
我们尝试通过聚合酶链反应（PCR）方法检测各种性染色体特异性序列，对多个法医样本进行性别和个体识别。样本包括30块附着在纱布上并储存了11个月至15年的血迹（15名男性、14名女性和1名性别不明者）以及10块漂白的白骨（死后8年至约10000年）。在已知性别的29块血迹中，与DXZ - 1重组的DYZ - 1（扩增DNA片段=149bp，Y染色体上约3000个拷贝）的性别判定率最高（29个样本中的28个），不过27个样本也可通过与DXZ - 1重组的DYZ - 1（扩增DNA片段=1000bp，Y染色体上约3000个拷贝）判定。与DXZ - 1重组的DYZ - 3（扩增DNA片段=170bp，Y染色体上约100个拷贝）的性别判定率相对较低（29个样本中的23个）。两个单一位点标记PAB或牙釉蛋白的性别判定率明显较低（分别为29个样本中的7个和9个）。27H39位点的性别判定率为29个样本中的27个；15名男性中的14名被判定（A = 186bp，1个；B = 190bp，7个；C = 194bp，5个；D = 198bp，1个；E = 202bp，0个）。一名性别不明者可通过多位点标记鉴定为男性，但除27H39位点外，无法通过任何单一位点标记鉴定。（摘要截断于250字）
