Wang K K, Costas P D, Bryan D J, Eby P L, Seckel B R
Department of Plastic and Reconstructive Surgery, Lahey Clinic, Burlington, Massachusetts, USA.
Microsurgery. 1995;16(2):65-70. doi: 10.1002/micr.1920160205.
The inside-out vein graft is a vein conduit pulled through itself to invert the normal orientation and place the adventitial layer within the lumen of the conduit. Our study compares regeneration of peripheral nerves in the rat through two conduits: inside-out graft of the jugular vein and autogenous nerve graft. In 10 rats, the right jugular vein was harvested, turned inside out, and used to bridge a 10 mm defect created in the right sciatic nerve. The 10 mm nerve segment from the right was then used as a standard nerve graft to bridge a 10 mm gap created in the left sciatic nerve. Rats were sacrificed at 8 and 12 weeks. Regeneration on the inside-out vein graft side showed superior functional results (faster conduction velocities) and improved histological results (greater axon counts) compared with the nerve grafted side. We feel the adventitial surface of the wall of the vein promotes nerve regeneration by providing an environment rich with collagen, laminin, and Schwann cells and promotes increased vascularization of the new nerve.
翻转静脉移植物是一种将静脉导管自身穿过以反转正常方向并将外膜层置于导管腔内的静脉导管。我们的研究通过两种导管比较大鼠周围神经的再生情况：颈静脉翻转移植物和自体神经移植物。在10只大鼠中，采集右侧颈静脉，翻转后用于桥接右侧坐骨神经中制造的10毫米缺损。然后将右侧的10毫米神经段用作标准神经移植物，以桥接左侧坐骨神经中制造的10毫米间隙。在8周和12周时处死大鼠。与神经移植侧相比，翻转静脉移植物侧的再生显示出更好的功能结果（更快的传导速度）和改善的组织学结果（更多的轴突计数）。我们认为静脉壁的外膜表面通过提供富含胶原蛋白、层粘连蛋白和雪旺细胞的环境促进神经再生，并促进新神经的血管化增加。
