Yoh M, Kawakami N, Funakoshi Y, Okada K, Honda T
Laboratory for Culture Collections, Research Institute for Microbial Diseases, Osaka University, Japan.
Microbiol Immunol. 1995;39(2):157-9. doi: 10.1111/j.1348-0421.1995.tb02183.x.
Reversed passive latex agglutination (RPLA) or enzyme-linked immunosorbent assay kits with beads (Bead-ELISA) are commercially available in Japan to detect the thermostable direct hemolysin (TDH) produced by Vibrio parahaemolyticus isolates. We evaluated whether these kits can be used to assay the pathogenic toxin, TDH-related hemolysin (TRH), produced by some so-called Kanagawa phenomenon-negative V. parahaemolyticus strains isolated from patients with diarrhea. Our results showed that the two kits, RPLA and Bead-ELISA, can detect TRH, although they were originally developed for detection of TDH. This may be due to the use of polyclonal anti-TDH antisera that cross react with TRH. Although the sensitivity for TDH detection by RPLA and Bead-ELISA differed tenfold, that for TRH detection was essentially equal. The minimum concentration of TRH required for detection by the two assay kits was about 10 ng/ml.
在日本，有用于检测副溶血性弧菌分离株产生的耐热直接溶血素（TDH）的反向被动乳胶凝集（RPLA）或带珠酶联免疫吸附测定试剂盒（Bead-ELISA）的商业产品。我们评估了这些试剂盒是否可用于检测从腹泻患者分离出的一些所谓神奈川现象阴性副溶血性弧菌菌株产生的致病性毒素TDH相关溶血素（TRH）。我们的结果表明，RPLA和Bead-ELISA这两种试剂盒虽然最初是为检测TDH而开发的，但都能检测TRH。这可能是由于使用了与TRH发生交叉反应的多克隆抗TDH抗血清。虽然RPLA和Bead-ELISA检测TDH的灵敏度相差十倍，但检测TRH的灵敏度基本相同。两种检测试剂盒检测TRH所需的最低浓度约为10 ng/ml。
