Madhusudan K, Ramesh V, Nagaraja V
Centre for Genetic Engineering, Indian Institute of Science, Bangalore.
Biochem Mol Biol Int. 1994 Jul;33(4):651-60.
We have recently reported the cloning of gyrA and gyrB genes from Mycobacterium tuberculosis H37Ra [Curr. Science, (1994) 66, 664-667]. Here, we present the complete nucleotide sequence of gyrB gene from M. tuberculosis H37Ra along with the flanking regions. The gyrA gene has been located 34 nucleotides downstream of gyrB and has been partially sequenced; both the genes seem to be transcribed from the promoter elements located upstream of gyrB coding sequence. The gyrB gene encodes a polypeptide of 714 amino acids. The deduced amino acid sequences of gyrB and a part of gyrA show extensive homology to the corresponding genes from other bacterial species. The DNA gyrase of M. tuberculosis could be utilised to develop new line of antitubercular drugs.
我们最近报道了从结核分枝杆菌H37Ra中克隆gyrA和gyrB基因[《当前科学》，(1994) 66, 664 - 667]。在此，我们给出结核分枝杆菌H37Ra的gyrB基因及其侧翼区域的完整核苷酸序列。gyrA基因位于gyrB下游34个核苷酸处，且已进行了部分测序；这两个基因似乎都从位于gyrB编码序列上游的启动子元件转录而来。gyrB基因编码一个由714个氨基酸组成的多肽。gyrB和部分gyrA推导的氨基酸序列与其他细菌物种的相应基因显示出广泛的同源性。结核分枝杆菌的DNA促旋酶可用于开发新的抗结核药物系列。
