Isobe Y, Muramatsu K
Department of Physiology, Nagoya City University Medical School, Japan.
Neurosci Lett. 1995 Mar 16;188(1):45-8. doi: 10.1016/0304-3940(95)11391-9.
To investigate the developmental features of peptide levels in the suprachiasmatic nucleus (SCN), the contents of vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP) and Arg-vasopressin (AVP) were measured in punched-out SCN tissue from rat pups on postnatal days (PD) 4, 15 and 20 using a specific enzyme immunoassay. The VIP content was high at night, and the day-night difference was largest at PD4 throughout the developmental stage. GRP and AVP contents increased following the maturation. GRP levels were higher during the dark than the light period from PD4 to PD20. Subjective day-night differences were also observed in blinded pups. Our results indicate that VIP, GRP and AVP in the SCN already show a diurnal rhythm in the SCN during the nursing period.
为了研究视交叉上核（SCN）中肽水平的发育特征，使用特异性酶免疫测定法，对出生后第4天、15天和20天的大鼠幼崽的SCN组织中血管活性肠肽（VIP）、胃泌素释放肽（GRP）和精氨酸加压素（AVP）的含量进行了测定。VIP含量在夜间较高，并且在整个发育阶段，昼夜差异在出生后第4天最大。GRP和AVP含量随着成熟而增加。从出生后第4天到第20天，GRP水平在黑暗期高于光照期。在失明的幼崽中也观察到了主观的昼夜差异。我们的结果表明，在哺乳期，SCN中的VIP、GRP和AVP已经呈现出昼夜节律。
