通过聚合酶链反应检测人类皮肤活检组织和犬滑膜液中的伯氏疏螺旋体DNA。
Detection of Borrelia burgdorferi DNA in human skin biopsies and dog synovial fluid by the polymerase chain reaction.
作者信息
Salinas-Meléndez J A, Tamez-González R, Welsh-Lozano O, Barrera-Saldaãna H A
机构信息
Departamento de Microbiología, F.M.V.Z. U.A.N.L., Monterrey, Mexico.
出版信息
Rev Latinoam Microbiol. 1995 Jan-Mar;37(1):7-10.
The polymerase chain reaction was used to amplify DNA sequences of the etiologic agent of Lyme disease, Borrelia burgdorferi, and was applied to the detection of the spirochete in humans and dogs. Oligonucleotide primers used in the reaction flank a 244-base-pair representing part of the variable region V4 of the B. burgdorferi 16S rRNA from biopsies of patients with acrodermatitis, and in synovial fluid from a dog with arthritis. These data suggest the presence of the disease in our state.
聚合酶链反应被用于扩增莱姆病病原体伯氏疏螺旋体的DNA序列，并应用于检测人和狗体内的螺旋体。反应中使用的寡核苷酸引物位于一段244个碱基对的侧翼，该碱基对代表来自肢端皮炎患者活检组织以及一只患有关节炎的狗的滑液中的伯氏疏螺旋体16S rRNA可变区V4的一部分。这些数据表明我们所在的州存在这种疾病。
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