运用反向线印迹法检测荷兰莱姆关节炎患者关节中的狭义伯氏疏螺旋体。
Detection of Borrelia burgdorferi sensu stricto by reverse line blot in the joints of Dutch patients with Lyme arthritis.
作者信息
van der Heijden I M, Wilbrink B, Rijpkema S G, Schouls L M, Heymans P H, van Embden J D, Breedveld F C, Tak P P
机构信息
Leiden University Medical Center, The Netherlands.
出版信息
Arthritis Rheum. 1999 Jul;42(7):1473-80. doi: 10.1002/1529-0131(199907)42:7<1473::AID-ANR22>3.0.CO;2-I.
OBJECTIVE
To analyze the presence of Borrelia burgdorferi sensu lato in synovial samples from the knee joint of patients with Lyme arthritis by polymerase chain reaction, and to differentiate the species by reverse line blot (RLB).
METHODS
Synovial fluid (SF) and synovial tissue (ST) samples were obtained from patients with Lyme arthritis (n = 4) and from patients with various other forms of arthritis (n = 9). DNA extracted from synovial samples was amplified by using, as a target, the spacer region between the 5S and 23S ribosomal RNA genes of B. burgdorferi sensu lato. Subsequently, 4 species-specific DNA probes were used in the RLB for specific hybridization.
RESULTS
DNA from B. burgdorferi sensu stricto DNA was detected in the SF and ST from 3 patients with Lyme arthritis. B. burgdorferi sensu lato DNA was not detected in the synovial samples from 9 control patients.
CONCLUSION
The relationship between different species of B. burgdorferi sensu lato and arthritis can be studied using direct analysis of extracted DNA from joint samples. This method can be used to study the association between particular clinical manifestations of Lyme disease and different species of B. burgdorferi sensu lato.
目的
通过聚合酶链反应分析莱姆关节炎患者膝关节滑膜样本中伯氏疏螺旋体狭义种的存在情况，并通过反向线印迹法（RLB）区分物种。
方法
从莱姆关节炎患者（n = 4）和其他各种形式关节炎患者（n = 9）获取滑液（SF）和滑膜组织（ST）样本。以伯氏疏螺旋体狭义种5S和23S核糖体RNA基因之间的间隔区为靶标，对从滑膜样本中提取的DNA进行扩增。随后，在反向线印迹法中使用4种物种特异性DNA探针进行特异性杂交。
结果
在3例莱姆关节炎患者的滑液和滑膜组织中检测到狭义伯氏疏螺旋体DNA。9例对照患者的滑膜样本中未检测到伯氏疏螺旋体广义种DNA。
结论
可通过直接分析关节样本中提取的DNA来研究伯氏疏螺旋体广义种不同物种与关节炎之间的关系。该方法可用于研究莱姆病特定临床表现与伯氏疏螺旋体广义种不同物种之间的关联。
Zotero 插件