Zhong F, Li X Y, Yang S L
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Yao Xue Xue Bao. 1995;30(2):93-7.
DADL, a delta-receptor selective enkephalin analogue, was prepared with the technique of solid-phase peptide synthesis. It was found to stimulate directly the proliferation of mouse splenic lymphocytes dose-dependently. However, synergistic effect of DADL at different concentrations with ConA and LPS were not found. In addition, TNF-alpha production was increased by ip DADL (1.0 mg.kg-1 x 6 d) in mice and the production of LPS-induced TNF-alpha in mouse peritoneal macrophages was enhanced after DADL was added in vitro. These results suggest that DADL, like enkephalins, exerts some immunoregulatory activities.
DADL（一种δ受体选择性脑啡肽类似物）采用固相肽合成技术制备。发现它能剂量依赖性地直接刺激小鼠脾淋巴细胞的增殖。然而，未发现不同浓度的DADL与刀豆蛋白A（ConA）和脂多糖（LPS）有协同作用。此外，腹腔注射DADL（1.0毫克·千克⁻¹×6天）可增加小鼠肿瘤坏死因子-α（TNF-α）的产生，并且在体外添加DADL后，小鼠腹腔巨噬细胞中LPS诱导的TNF-α产生增强。这些结果表明，DADL与脑啡肽一样，具有一些免疫调节活性。
