Poverennĭ A M, Podgorodnichenko V K, Tsypliakovskaia L M, Potapova N V
Mol Biol (Mosk). 1975 Nov-Dec;9(6):828-35.
The conditions were developed to measure antibodies to denatured and native DNA. It was shown that membrane filters treated with alkali adsorbed denatured DNA complexed with antibodies. Utilizing this method it was shown that antibodies to denatured DNA react with pyrimidines (probably thymine). This assay provides proof of the wide existence antibodies to native DNA in the sera of patients with systemic lupus erythematosus. DNA-binding of sera was significantly depended on reaction condition.
这些条件是为了检测针对变性和天然DNA的抗体而制定的。结果表明，经碱处理的膜滤器可吸附与抗体结合的变性DNA。利用该方法发现，针对变性DNA的抗体与嘧啶（可能是胸腺嘧啶）发生反应。该检测方法证明了系统性红斑狼疮患者血清中广泛存在针对天然DNA的抗体。血清的DNA结合显著依赖于反应条件。
