Anthony A, Dhillon A P, Fidler H, McFadden J, Billington O, Nygard G, Pounder R E, Wakefield A J
University Department of Histopathology, Royal Free Hospital School of Medicine, London, UK.
Int J Exp Pathol. 1995 Apr;76(2):149-55.
We report mycobacterial granulomatous inflammation in the ulcerated caecum of rats that received indomethacin. Two groups of male rats were treated with dietary indomethacin 3 mg/kg/day or untreated diet for 3 weeks. Six out of 8 indomethacin treated rats showed both ulceration and inflammation of the caecal mucosa. Two of the rats showing caecal ulceration also showed distinct granulomatous inflammation of the caecal mucosa and acid-fast bacilli were identified within granulomata. None of the other indomethacin treated or control rats contained acid-fast bacilli within caecal tissue sections but they were present, in the same sections, within the lumen of most rats in both groups. Polymerase chain reaction analysis identified the mycobacterial 65 kDa GroEL gene within control and granulomatous caecal tissue. In a repeat of indomethacin administration to a third group of rats, culture of both non-granulomatous caecal tissue (containing histologically identified luminal acid-fast bacilli) and faecal samples for mycobacteria was negative.
我们报告了在接受吲哚美辛治疗的大鼠溃疡盲肠中出现的分枝杆菌性肉芽肿性炎症。两组雄性大鼠分别给予含3 mg/kg/天吲哚美辛的饮食或未处理饮食，持续3周。8只接受吲哚美辛治疗的大鼠中有6只出现盲肠黏膜溃疡和炎症。2只出现盲肠溃疡的大鼠还表现出盲肠黏膜明显的肉芽肿性炎症，并且在肉芽肿内发现了抗酸杆菌。在其他接受吲哚美辛治疗的大鼠或对照大鼠的盲肠组织切片中均未发现抗酸杆菌，但在两组大多数大鼠的相同切片的肠腔内均有抗酸杆菌存在。聚合酶链反应分析在对照和肉芽肿性盲肠组织中均鉴定出分枝杆菌65 kDa GroEL基因。在对第三组大鼠重复给予吲哚美辛的实验中，对非肉芽肿性盲肠组织（组织学鉴定含有肠腔抗酸杆菌）和粪便样本进行分枝杆菌培养均为阴性。
