对两个法国家庭的克罗恩病进行的深入研究。
An in-depth study of Crohn's disease in two French families.
作者信息
Van Kruiningen H J, Colombel J F, Cartun R W, Whitlock R H, Koopmans M, Kangro H O, Hoogkamp-Korstanje J A, Lecomte-Houcke M, Devred M, Paris J C
机构信息
Department of Pathobiology, University of Connecticut, Storrs.
出版信息
Gastroenterology. 1993 Feb;104(2):351-60. doi: 10.1016/0016-5085(93)90401-w.
BACKGROUND
Two French families were investigated. In the first a husband, wife, and 4 children had Crohn's disease; in the second 7 of 11 children had the disease. There was no history of Crohn's disease in antecedent generations and no linkage to HLA haplotypes.
METHODS
Methods included family interviews; review of medical records, radiographs, and pathology slides; serology; selective stool culture; enzyme-linked immunosorbent assay for fecal viral detection; and immunocytochemistry.
RESULTS
In both families multiple cases occurred among siblings in 7-13-month periods. There appeared to be a 4-8-year recurrence of new disease in both families. Radiographs showed a remarkable similarity in the pattern of disease, confined to distal ileum and cecum, in the members of family 1. Examination for pathology showed granulomas in all 8 patients for whom tissues were available. Acid-fast organisms or Campylobacter-like organisms were not found in tissue sections, and immunocytochemistry was negative for mycobacteria and Yersinia. Stool cultures were negative for mycobacteria, Yersinia, and Mycoplasma. Torovirus and coronavirus antigens were not found in stool. Serology was negative for antibodies to Brucella, Yersinia, influenza, and three enteropathogenic viruses of animals.
CONCLUSIONS
The circumstances and data suggest that an infectious microorganism is responsible for these clusterings of Crohn's disease.
背景
对两个法国家庭进行了调查。在第一个家庭中，丈夫、妻子和4个孩子患有克罗恩病；在第二个家庭中，11个孩子中有7个患有该病。先辈中没有克罗恩病病史，且与HLA单倍型无连锁关系。
方法
方法包括家庭访谈；查阅病历、X光片和病理切片；血清学检查；选择性粪便培养；用于粪便病毒检测的酶联免疫吸附测定；以及免疫细胞化学。
结果
在两个家庭中，兄弟姐妹在7至13个月期间均出现多例病例。两个家庭中似乎都有新发病例4至8年的复发情况。X光片显示，在家庭1的成员中，疾病模式有显著相似性，局限于回肠末端和盲肠。病理检查显示，在所有8例可获取组织的患者中均发现肉芽肿。在组织切片中未发现抗酸菌或弯曲杆菌样生物体，免疫细胞化学检测显示分枝杆菌和耶尔森菌呈阴性。粪便培养显示分枝杆菌、耶尔森菌和支原体呈阴性。粪便中未发现环曲病毒和冠状病毒抗原。血清学检测显示，布鲁氏菌、耶尔森菌、流感以及三种动物肠道致病病毒的抗体呈阴性。
结论
这些情况和数据表明，一种传染性微生物是这些克罗恩病聚集病例的病因。
Zotero 插件