Chant I D, Rose P E, Morris A G
Department of haematology, South Warwickshire Hospital.
Br J Haematol. 1995 May;90(1):163-8. doi: 10.1111/j.1365-2141.1995.tb03395.x.
Expression of heat-shock proteins (hsp) was analysed in the leukaemic cells of 12 patients with acute myeloid leukaemia (AML) and nine patients with chronic myeloid leukaemia (CML). Using monoclonal antibodies to hsp70, hsp90 and hsp60 (ML30, a mycobacterial antigen with homology to human hsp60), we measured hsp levels by flow cytometry of permeabilized cells. Mononuclear cells from 10 healthy volunteers were also examined. The results demonstrate that hsp expression is significantly increased (P < 0.01) in the circulating cells of patients with AML compared with cells from CML patients, and compared with normal peripheral blood mononuclear cells. This increased pattern of expression was found for all three heat-shock protein families included in this study. Mononuclear cells from leukaemic patients showed a heterogenous pattern of hsp expression, between different patients, between cells from individual patients, and between the different hsp proteins examined. It is possible that hsp expression relates to the differentiation state or proliferative potential of these leukaemic cells.
对12例急性髓系白血病（AML）患者和9例慢性髓系白血病（CML）患者的白血病细胞中热休克蛋白（hsp）的表达进行了分析。使用针对hsp70、hsp90和hsp60（ML30，一种与人类hsp60具有同源性的分枝杆菌抗原）的单克隆抗体，我们通过对透化细胞进行流式细胞术来测量hsp水平。还检测了10名健康志愿者的单核细胞。结果表明，与CML患者的细胞以及正常外周血单核细胞相比，AML患者循环细胞中的hsp表达显著增加（P < 0.01）。在本研究纳入的所有三个热休克蛋白家族中均发现了这种增加的表达模式。白血病患者的单核细胞在不同患者之间、个体患者的细胞之间以及所检测的不同hsp蛋白之间显示出hsp表达的异质性模式。hsp表达可能与这些白血病细胞的分化状态或增殖潜能有关。
