Ohara M, Hibi T, Watanabe N, Kobayashi K, Takaishi H, Hayashi A, Hosoda Y, Toda K, Iwao Y, Watanabe M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
J Gastroenterol Hepatol. 1995 Mar-Apr;10(2):158-64. doi: 10.1111/j.1440-1746.1995.tb01071.x.
Immunoglobulin G (IgG) subclasses of anticolon antibodies were studied in patients with ulcerative colitis (UC) using enzyme-linked immunosorbent assay (ELISA). The concentrations of total serum IgG subclasses were also measured by ELISA. The values for total serum IgG subclasses in patients with UC were not significantly different from those in normal controls, while the ratio of IgG1 to IgG2 in the patients was significantly higher than that in normal controls. All four IgG subclasses of autoantibodies were demonstrated in the sera of the patients. IgG4 anticolon antibodies were detected most frequently (15 out of 18 patients, 83%). IgG2 was the next most prevalent (9 of 18 patients, 50%). The activity of anticolon antibodies in each subclass did not correlate with the concentration of the corresponding serum IgG subclass. Seven cell lines producing anticolon antibodies were obtained from the colonic mucosa of the patients by Epstein-Barr virus (EBV) transformation. IgG subclasses of anticolon antibodies secreted by these cell lines were also varied. IgG4 subclass was secreted by three EBV transformed cell lines, all of which produced IgG4 anticolon antibodies. These results suggest that all four different IgG subclasses could respond to the colon antigens and that various antigens in colonic mucosa or lumen may contribute to the induction of those autoantibodies. In addition, the prominence of IgG4 anticolon antibodies may support the pathogenic role of this subclass in UC as in other autoimmune diseases.
采用酶联免疫吸附测定法（ELISA）对溃疡性结肠炎（UC）患者抗结肠抗体的免疫球蛋白G（IgG）亚类进行了研究。同时也通过ELISA检测了血清总IgG亚类的浓度。UC患者血清总IgG亚类的值与正常对照组相比无显著差异，而患者中IgG1与IgG2的比值显著高于正常对照组。在患者血清中检测到了所有四种自身抗体的IgG亚类。IgG4抗结肠抗体检测频率最高（18例患者中有15例，83%）。其次是IgG2（18例患者中有9例，50%）。各亚类抗结肠抗体的活性与相应血清IgG亚类的浓度无关。通过爱泼斯坦-巴尔病毒（EBV）转化从患者结肠黏膜中获得了7株产生抗结肠抗体的细胞系。这些细胞系分泌的抗结肠抗体的IgG亚类也各不相同。有3株EBV转化细胞系分泌IgG4亚类，它们均产生IgG4抗结肠抗体。这些结果表明，所有四种不同的IgG亚类均可对结肠抗原产生反应，结肠黏膜或肠腔内的各种抗原可能参与了这些自身抗体的诱导。此外，IgG4抗结肠抗体的突出表现可能支持该亚类在UC中与其他自身免疫性疾病一样具有致病作用。
