Kim S J, Han D, Moon K D, Rhee J S
Agricultural Product Utilization Division, Korea Food Research Institute, Kyonggi.
Biosci Biotechnol Biochem. 1995 May;59(5):822-6. doi: 10.1271/bbb.59.822.
The superoxide dismutase (SOD)-like activity of natural antioxidants was evaluated by measuring the inhibition of pyrogallol autoxidation that is catalyzed by the superoxide radical. Among 22 water-soluble antioxidants tested, L-ascrobic acid, L-ascorbic acid 6-palmitate, glutathione (reduced form), (+)-catechin, and (-)-epicatechin showed effective SOD-like activity. To analyze lipophilic antioxidants, an optically clear organic system composed of diethyl ether, surfactant (dioctyl sulfosuccinate, AOT) and water, called reverse micelles, was developed. The optimum concentrations of AOT, water and pyrogallol for determining SOD-like activity were found to be 50 mM, 1.3 M, and 40 mM, respectively. After proving that pyrogallol autoxidation was mediated by the superoxide anion in that system, the SOD-like activity of 24 lipophilic antioxidants was measured. Cinnamon oil, gamma-oryzanol, extract of rosemary leaf, L-alpha-lecithin, and L-alpha-cephalin exhibited activity, although the activity of some antioxidants could not be measured because of the intense color or low solubility.
通过测量超氧阴离子催化的邻苯三酚自氧化的抑制作用，评估了天然抗氧化剂的超氧化物歧化酶（SOD）样活性。在测试的22种水溶性抗氧化剂中，L-抗坏血酸、L-抗坏血酸6-棕榈酸酯、谷胱甘肽（还原型）、（+）-儿茶素和（-）-表儿茶素表现出有效的SOD样活性。为了分析亲脂性抗氧化剂，开发了一种由乙醚、表面活性剂（二辛基磺基琥珀酸酯，AOT）和水组成的光学透明有机体系，称为反胶束。发现用于测定SOD样活性的AOT、水和邻苯三酚的最佳浓度分别为50 mM、1.3 M和40 mM。在证明该体系中邻苯三酚自氧化由超氧阴离子介导后，测定了24种亲脂性抗氧化剂的SOD样活性。肉桂油、γ-谷维素、迷迭香叶提取物、L-α-卵磷脂和L-α-脑磷脂表现出活性，不过由于颜色过深或溶解度低，一些抗氧化剂的活性无法测定。
