Coursaget P, Maupas P, Larbaigt G, Raynaud B, Grenier B
Pathol Biol (Paris). 1976 Mar;24(3):227-31.
Auto-analysis has been adapted to the study of complement fixation on flu antigen-antibody complexes. The antigen used consists of a complete virus of strain A2/AICHI/68/H3N2 (antigen of envelope V). The advantages of this method reside in the reproducibility of the results and in instrumental reading with graphic recording and conservation of a graphical result. The recording of the percentage of hemolysis given by the auto-analyser permits calculation of the rate of the reaction K37 of complement fixation on the formed immune complex. This value is independent of temperature, time of fixation and the initial quantity of complement used. It is proportional to the quantity of specific antibody. Thus, the value of K37 is proposed as mode of expression of the results replacing the limit serum dilution.
自动分析已应用于流感抗原-抗体复合物补体结合的研究。所使用的抗原由A2/爱知/68/H3N2株的完整病毒（包膜V抗原）组成。该方法的优点在于结果的可重复性以及仪器读取、图形记录和图形结果保存。自动分析仪给出的溶血百分比记录允许计算补体结合到形成的免疫复合物上的反应K37速率。该值与温度、结合时间和所用补体的初始量无关。它与特异性抗体的量成正比。因此，建议用K37值作为结果的表达模式，取代血清稀释极限。
