Coursaget P, Maupas P, Vargues R
Ann Microbiol (Paris). 1976 Aug-Sep;127B(2):269-79.
A kinetic study of the fixation of complement by the influenza immune complex was performed, using an automatic method originally described by Vargues for use in immunobacteriology. The autoanalyser provided curves which could be transformed into a simple equation. The experimental graph of the complement fixation reaction is superposable on a model curve obtained by exponential dilurion of complement. The methematical formula of this exponential complement dilution fits results of complement fixation by the immunecomplex. The rate constant can be estimated from the tangent at the orgin of the curve; it is a function of the nature of the immunesystem, the quantity of immune-complex and the temperature. These results show that the reaction behaves according to the equations of first order kinetics. This law is therefore the same as that described for other immunological systems, such as the seroneutralization of bacteriophage or the fixation of isoagglutinins on red cells. The kinetic study leads to the proposal of the k37 value for the titration of an anti-influenza serum. It is the rate constant of the reaction at 37 degrees C and is independant of the time and temperature of the fixation system as it is of the initial quantity of complement. Increased sensitivity of the reaction does not yield by higher values of k37 but affords greater precision in its calculation. This approach is identical to the one proposed by Adams for the titration of anti-phage sera.
利用瓦尔格斯最初描述的用于免疫细菌学的自动方法，对流感免疫复合物固定补体进行了动力学研究。自动分析仪提供的曲线可转化为一个简单方程。补体固定反应的实验曲线与通过补体指数稀释获得的模型曲线可叠加。这种指数补体稀释的数学公式符合免疫复合物固定补体的结果。速率常数可从曲线原点处的切线估算得出；它是免疫系统性质、免疫复合物数量和温度的函数。这些结果表明该反应符合一级动力学方程。因此，该规律与其他免疫学系统所描述的规律相同，如噬菌体的血清中和反应或红细胞上同种凝集素的固定反应。动力学研究提出了抗流感血清滴定的k37值。它是37摄氏度时反应的速率常数，与固定系统的时间和温度以及补体的初始量无关。反应灵敏度的提高并非通过更高的k37值实现，而是在其计算中提供了更高的精度。这种方法与亚当斯提出的抗噬菌体血清滴定方法相同。
