de Oliveira J A, Bráulio V B, Santos G C, Scandar M P, Freitas O, Zucoloto S
Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Cell Mol Biol (Noisy-le-grand). 1995 Mar;41(2):227-32.
Literature reports that chronically ingested ethanol induces changes in the morphology of the small bowel mucous membranes. It has a topical toxic effect on the epithelium of the proximal jejunum and a blood-borne effect on the epithelium of the ileum because its absorption is almost complete in the stomach, duodenum and proximal jejunum. In addition there are also reports showing stimulation of enterocyte proliferation after segmental intestine resection. In this report we compare a group of rats submitted to resection of the proximal jejunum and fed a liquid diet containing 35% of the total calories intake as ethanol for four weeks to its control pair-fed group. In both groups we studied the mucosal alkaline phosphatase (APase) activity by histochemical as well as biochemical methods. We found a decreased APase activity in the homogenate of the intestinal mucous membrane in the alcoholic group and a reduced enzymatic activity in the brush border of the ileum enterocytes, as demonstrated by histochemical qualitative and densitometric assays. The result suggests that this change in APase activity of the brush border may represent enterocyte immaturity induced by long-standing ethanol intake in the remnant ileum after proximal resection.
文献报道，长期摄入乙醇会导致小肠黏膜形态发生变化。它对空肠近端上皮有局部毒性作用，对回肠上皮有血源性影响，因为其在胃、十二指肠和空肠近端几乎完全吸收。此外，也有报道显示，节段性肠切除术后肠上皮细胞增殖受到刺激。在本报告中，我们将一组接受空肠近端切除并以含总热量摄入35%乙醇的流质饮食喂养四周的大鼠与其对照配对喂养组进行比较。在两组中，我们通过组织化学和生化方法研究了黏膜碱性磷酸酶（APase）活性。通过组织化学定性和光密度测定分析表明，我们发现酒精组肠黏膜匀浆中APase活性降低，回肠肠上皮细胞刷状缘的酶活性降低。结果表明，刷状缘APase活性的这种变化可能代表近端切除术后残留回肠中因长期摄入乙醇导致的肠上皮细胞不成熟。
