Al-Watban F A, Zhang X Y
Laser Research, Biological, & Medical Research Department, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.
J Clin Laser Med Surg. 1997;15(5):209-15. doi: 10.1089/clm.1997.15.209.
We compared the wound healing process on Sprague-Dawley rats between Argon Laser and Krypton Laser, and calculated the laser actual doses after correction on wound healing on rats and estimated the clinical doses on wound healing for human skin.
Laboratory work provided some support for the use of low-intensity laser radiation in wound healing. Some studies found that laser irradiation may either enhance, inhibit, or has no effect on the function of a variety of microorganisms and cells. Animal studies also offered some basis for treatment. Improvements, particularly in the earliest phases of wound healing, have been reported following laser irradiation.
We used Argon Laser (488-514 nm) and Krypton Laser (670 nm) in the study. The laser beam was delivered through a system of fiber optic in Argon Laser and reflector in Krypton Laser. The rats treated were restrained in a Plexiglas cage without anesthesia during the laser irradiation period. The percentage of the wound healing acceleration in days and size, actual doses, and estimated clinical doses were calculated as follows: AccD = (1-TD/CD) x 100%, AccS = (1-TA/CA) x 100%, AD = D(in) - D(gl) - D(SR) and ECD = AD = D(sr), respectively.
The acceleration effects of wound healing in days (AccD) were 22.93% and 14.54%, size reductions (AccS) were 41.93% and 30.41% at the optimal stimulative incident dose of 20 J/cm2. Zero bioactivation shown at the incident doses of 80 J/cm2 and 100 J/cm2. The inhibitory effects of wound healing in days were -7.72% and -3.37%, in size reduction were -13.35% and -12.88% at the maximal inhibitory incident dose of 140 J/cm2 for Argon and Krypton Lasers, respectively. The actual doses were 5.21 J/cm2 and 4.03 J/cm2, the estimated clinical doses were 5.50 J/cm2 and 4.25 J/cm2 at optimal stimulative incident dose 20 J/cm2 for Argon and Krypton Lasers, respectively.
Low power laser therapy at the appropriate dosimetric parameters can provide the acceleration effects of wound healing on rats. The effects were dependent with the doses and laser wavelengths used. In this experiment, the optimum stimulative dose was 20 J/cm2 and the Argon Laser with 488 nm was more effective than the Krypton Laser with 670 nm. The zero bioactivation and inhibition effect of wound healing on rats occurred in Argon Laser and Krypton Laser.
我们比较了氩激光和氪激光对Sprague-Dawley大鼠伤口愈合过程的影响，并在对大鼠伤口愈合情况进行校正后计算了激光实际剂量，同时估算了人体皮肤伤口愈合的临床剂量。
实验室研究为低强度激光辐射在伤口愈合中的应用提供了一些支持。一些研究发现激光照射可能增强、抑制或对多种微生物和细胞的功能无影响。动物研究也为治疗提供了一些依据。据报道，激光照射后伤口愈合有改善，尤其是在愈合的早期阶段。
我们在研究中使用了氩激光（488 - 514 nm）和氪激光（670 nm）。氩激光通过光纤系统传输光束，氪激光通过反射器传输光束。在激光照射期间，将接受治疗的大鼠置于有机玻璃笼中，不进行麻醉。伤口愈合加速的天数和大小百分比、实际剂量以及估算的临床剂量计算如下：加速天数（AccD）=（1 - TD/CD）×100%，加速大小（AccS）=（1 - TA/CA）×100%，实际剂量（AD）= D（in）- D（gl）- D（SR），估算临床剂量（ECD）= AD = D（sr）。
在最佳刺激入射剂量20 J/cm²时，伤口愈合加速天数（AccD）分别为22.93%和14.54%，伤口大小减小（AccS）分别为41.93%和30.41%。在入射剂量80 J/cm²和100 J/cm²时未显示生物激活作用。在最大抑制入射剂量140 J/cm²时，氩激光和氪激光对伤口愈合天数的抑制作用分别为-7.72%和-3.37%，对伤口大小减小的抑制作用分别为-13.35%和-12.88%。在最佳刺激入射剂量20 J/cm²时，氩激光和氪激光的实际剂量分别为5.21 J/cm²和4.03 J/cm²，估算临床剂量分别为5.50 J/cm²和4.25 J/cm²。
在适当的剂量参数下进行低功率激光治疗可对大鼠伤口愈合产生加速作用。这些作用取决于所用的剂量和激光波长。在本实验中，最佳刺激剂量为20 J/cm²，488 nm的氩激光比670 nm的氪激光更有效。氩激光和氪激光在大鼠伤口愈合中出现了零生物激活和抑制作用。
