Magnol J P, Fournel C, Marchal T, Chabanne L, Bryon P A, Felman P
Ecole Vétérinaire de Lyon.
Bull Acad Natl Med. 1995 Jan;179(1):51-65.
Among the non-Hodgkin's malignant lymphomas of the dog, which are largely dominated by the centroblastic heterogeneous type, there is an original form of malignant lymphoma which is homogeneous and diffuse, with macronucleolated medium-sized cells. These cells seem to be morphologically very similar to those which constitute the majority population in the marginal zone of the secondary follicle of the lymph node in the dog, and which appear in the course of certain conditions: systemic lupus erythematosus, leishmaniasis, satellite lymph nodes in benign or malignant tumors. The aim of this study was twofold: on the one hand to establish, in the canine species, the identity of the lymphomatous cells and the reactive cells that make up the marginal zone, i.e. the filiation between the hyperplastic marginal zones and the macronucleolated malignant lymphoma with medium-sized cells, and, on the other hand, to compare this type of malignant lymphoma with those which are reputed to originate in the marginal zone in humans, for example the malignant lymphoma of the lymphoid tissue associated with the mucous membranes, and the monocytoid malignant B-cell lymphomas. Ninety four malignant lymphomas were observed between 1989 and 1994 at the Veterinary School in Lyon; these consisted of 71 cases showing medium or high-grade malignancy, 17 cases with small cells, of low-grade malignancy, and 6 cases of mycosis fungoides. Among the 71 cases of medium and high-grade malignancy, 8 were immunoblastic, 5 centroblastic homogeneous, 50 centroblastic heterogeneous, and 8 homogeneous with macronucleolated medium-sized cells. The methods used in these 94 cases were of a morphological type: cytology, histology, transmission microscopy and immunohistochemistry. The cytohistological, ultrastructural and immuno-phenotypical characteristics (CD3-, CIg-, Ki-67- phenotype) of the lymphomatous cells and the cells of the marginal zone were found to be identical, in the dog; this strongly suggests B-lineage cells which do not secrete cytoplasmic immunoglobulins and are not involved in the cell cycle. Finally, these cells seem to us to be morphologically very similar to the minority population described by Van den Oord in the marginal zone of the secondary follicles in the lymph node in humans, in certain reactive situations.
在犬类非霍奇金恶性淋巴瘤中，主要以中心母细胞异质性类型为主，存在一种原始形式的恶性淋巴瘤，其具有均一性和弥漫性，细胞为大核仁的中等大小细胞。这些细胞在形态上似乎与构成犬淋巴结次级滤泡边缘区大多数细胞群的细胞非常相似，且在某些情况下出现：系统性红斑狼疮、利什曼病、良性或恶性肿瘤中的卫星淋巴结。本研究的目的有两个：一方面，在犬类中确定构成边缘区的淋巴瘤细胞和反应性细胞的一致性，即增生性边缘区与大核仁中等大小细胞的恶性淋巴瘤之间的谱系关系；另一方面，将这种类型的恶性淋巴瘤与人类中据称起源于边缘区的淋巴瘤进行比较，例如与黏膜相关的淋巴组织恶性淋巴瘤和单核细胞样恶性B细胞淋巴瘤。1989年至1994年期间，在里昂兽医学院观察到94例恶性淋巴瘤；其中71例为中或高度恶性，17例为小细胞、低度恶性，6例为蕈样霉菌病。在71例中高度恶性病例中，8例为免疫母细胞型，5例为中心母细胞均一型，50例为中心母细胞异质性型，8例为大核仁中等大小细胞均一型。这94例病例所采用的方法为形态学类型：细胞学、组织学、透射显微镜检查和免疫组织化学。在犬类中发现淋巴瘤细胞和边缘区细胞的细胞组织学、超微结构和免疫表型特征（CD3 -、CIg -、Ki - 67表型）是相同的；这强烈提示为不分泌细胞质免疫球蛋白且不参与细胞周期的B系细胞。最后，在我们看来，这些细胞在形态上与范登奥德描述的人类淋巴结次级滤泡边缘区在某些反应性情况下的少数细胞群非常相似。
