Severson D W, Thathy V, Mori A, Zhang Y, Christensen B M
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
Genetics. 1995 Apr;139(4):1711-7. doi: 10.1093/genetics/139.4.1711.
Susceptibility of the mosquito Aedes aegypti to the malarial parasite Plasmodium gallinaceum was investigated as a quantitative trait using restriction fragment length polymorphisms (RFLP). Two F2 populations of mosquitoes were independently prepared from pairwise matings between a highly susceptible and a refractory strain of A. aegypti. RFLP were tested for association with oocyst development on the mosquito midgut. Two putative quantitative trait loci (QTL) were identified that significantly affect susceptibility. One QTL, pgs[2,LF98], is located on chromosome 2 and accounted for 65 and 49% of the observed phenotypic variance in the two populations, respectively. A second QTL, pgs[3,MalI], is located on chromosome 3 and accounted for 14 and 10% of the observed phenotypic variance in the two populations, respectively. Both QTL exhibit a partial dominance effect on susceptibility, wherein the dominance effect is derived from the refractory parent. No indication of epistasis between these QTL was detected. Evidence suggests that either a tightly linked cluster of independent genes or a single locus affecting susceptibility to various mosquito-borne parasites and pathogens has evolved near the LF98 locus; in addition to P. gallinaceum susceptibility, this general genome region has previously been implicated in susceptibility to the filarial nematode Brugia malayi and the yellow fever virus.
利用限制性片段长度多态性（RFLP）作为一种数量性状，研究了埃及伊蚊对疟原虫鸡疟原虫的易感性。从埃及伊蚊的一个高易感性品系和一个抗性品系之间的成对交配中独立制备了两个F2代蚊虫群体。测试RFLP与蚊虫中肠上卵囊发育的关联性。鉴定出两个显著影响易感性的假定数量性状基因座（QTL）。一个QTL，pgs[2,LF98]，位于2号染色体上，分别占两个群体中观察到的表型变异的65%和49%。第二个QTL，pgs[3,MalI]，位于3号染色体上，分别占两个群体中观察到的表型变异的14%和10%。两个QTL对易感性均表现出部分显性效应，其中显性效应源自抗性亲本。未检测到这些QTL之间存在上位性的迹象。有证据表明，在LF98基因座附近，要么进化出了一个紧密连锁的独立基因簇，要么存在一个影响对各种蚊媒寄生虫和病原体易感性的单个基因座；除了对鸡疟原虫的易感性外，这个一般的基因组区域先前还与对丝虫线虫马来布鲁线虫和黄热病病毒的易感性有关。
