Severson D W, Mori A, Zhang Y, Christensen B M
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison.
Am J Trop Med Hyg. 1994 Apr;50(4):425-32. doi: 10.4269/ajtmh.1994.50.425.
Restriction fragment length polymorphism (RFLP) markers derived from the yellow fever mosquito, Aedes aegypti, were used in hybridizations to genomic DNA of the following mosquito species: Ae. albopictus, Ae. togoi, Armigeres subalbatus, Culex pipiens, and Anopheles gambiae. Interspecific hybridization with Ae. aegypti probes varied from 50% (An. gambiae) to 100% (Ae. albopictus) under high stringency conditions. We demonstrated the usefulness of using RFLP profiles to examine genetic diversity between mosquito populations; Ae. aegypti RFLP markers were used to examine genetic relatedness between 10 laboratory strains of Ae. aegypti as well as between nine populations representing four Cx. pipiens subspecies. These results indicate that many Ae. aegypti RFLP markers should have direct applicability in gaining a better understanding of genome structure in other mosquito species, including RFLP linkage mapping and determinations of genetic relatedness among field populations.
从黄热病蚊子埃及伊蚊衍生而来的限制性片段长度多态性（RFLP）标记，被用于与以下蚊子物种的基因组DNA进行杂交：白纹伊蚊、东乡伊蚊、骚扰阿蚊、致倦库蚊和冈比亚按蚊。在高严谨条件下，与埃及伊蚊探针的种间杂交率从50%（冈比亚按蚊）到100%（白纹伊蚊）不等。我们证明了使用RFLP图谱来检查蚊子种群间遗传多样性的有效性；埃及伊蚊RFLP标记被用于检查10个埃及伊蚊实验室品系之间以及代表四个致倦库蚊亚种的九个种群之间的遗传相关性。这些结果表明，许多埃及伊蚊RFLP标记在更好地理解其他蚊子物种的基因组结构方面应具有直接适用性，包括RFLP连锁图谱绘制和田间种群遗传相关性的测定。
