Kipersztok S, Osawa G A, Liang L F, Modi W S, Dean J
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genomics. 1995 Jan 20;25(2):354-9. doi: 10.1016/0888-7543(95)80033-i.
Human POM-ZP3 is a novel bipartite RNA transcript that is derived from a gene homologous to rat POM121 (a nuclear pore membrane protein) and ZP3 (a sperm receptor ligand in the zona pellucida). The 5' region is 77% identical to the 5' end of the coding region of rat POM121 and appears to represent a partial duplication of a gene encoding a human homologue of this rodent gene. The 3' end of the POM-ZP3 transcript is 99% identical to ZP3 and appears to have arisen from a duplication of the last four exons (exons 5-8) of ZP3. Using Northern blots and RT-PCR, POM-ZP3 transcripts were detected in human ovaries, testes, spleen, thymus, lymphocytes, prostate, and intestines. The longest open reading frame encodes a conceptual protein of 210 amino acids, the first 76 of which are 83% identical to residues 241-315 of rat POM121. The next 125 amino acids are 98% identical to residues 239-363 of the 424-amino-acid human ZP3 protein. By fluorescence in situ hybridization, genomic fragments of ZP3 and a human homologue of POM121 were localized to chromosome 7q11.23. Taken together, these data suggest that partial duplications of human ZP3 and a POM121-like gene have resulted in a fusion transcript, POM-ZP3, that is expressed in multiple human tissues.
人POM-ZP3是一种新型的双分体RNA转录本，它来源于一个与大鼠POM121（一种核孔膜蛋白）和ZP3（透明带中的精子受体配体）同源的基因。5'区域与大鼠POM121编码区的5'端有77%的同一性，似乎代表了一个编码该啮齿动物基因人类同源物的基因的部分重复。POM-ZP3转录本的3'端与ZP3有99%的同一性，似乎是由ZP3的最后四个外显子（外显子5-8）的重复产生的。使用Northern印迹法和RT-PCR，在人卵巢、睾丸、脾脏、胸腺、淋巴细胞、前列腺和肠道中检测到了POM-ZP3转录本。最长的开放阅读框编码一个210个氨基酸的概念性蛋白质，其中前76个氨基酸与大鼠POM121的241-315位残基有83%的同一性。接下来的125个氨基酸与424个氨基酸的人ZP3蛋白的239-363位残基有98%的同一性。通过荧光原位杂交，ZP3和POM121的人类同源物的基因组片段被定位到7号染色体q11.23区域。综上所述，这些数据表明，人类ZP3和一个类似POM121的基因的部分重复产生了一个融合转录本POM-ZP3，它在多种人类组织中表达。
