采用血沉棕黄层寄生虫培养法诊断人类免疫缺陷病毒感染患者的内脏利什曼病
Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients.
作者信息
López-Vélez R, Laguna F, Alvar J, Pérez-Molina J A, Molina R, Martinez P, Villarrubia J
机构信息
Tropical Medicine and Clinical Parasitology Unit, Hospital Ramón y Cajal, Madrid, Spain.
出版信息
J Clin Microbiol. 1995 Apr;33(4):937-9. doi: 10.1128/jcm.33.4.937-939.1995.
Abstract
Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.
摘要
从25例经骨髓穿刺抽吸物（涂片和培养）确诊为内脏利什曼病的人类免疫缺陷病毒阳性患者的外周血中采集两份血沉棕黄层样本，接种于施奈德培养基和诺维-麦克尼尔-尼科尔培养基上进行培养。血培养阳性率为67%。平均生长时间为10天。这是一种简便、无创且敏感的技术。
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