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采用血沉棕黄层寄生虫培养法诊断人类免疫缺陷病毒感染患者的内脏利什曼病

Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients.

作者信息

López-Vélez R, Laguna F, Alvar J, Pérez-Molina J A, Molina R, Martinez P, Villarrubia J

机构信息

Tropical Medicine and Clinical Parasitology Unit, Hospital Ramón y Cajal, Madrid, Spain.

出版信息

J Clin Microbiol. 1995 Apr;33(4):937-9. doi: 10.1128/jcm.33.4.937-939.1995.

DOI:10.1128/jcm.33.4.937-939.1995
PMID:7790464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228071/
Abstract

Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.

摘要

从25例经骨髓穿刺抽吸物（涂片和培养）确诊为内脏利什曼病的人类免疫缺陷病毒阳性患者的外周血中采集两份血沉棕黄层样本，接种于施奈德培养基和诺维-麦克尼尔-尼科尔培养基上进行培养。血培养阳性率为67%。平均生长时间为10天。这是一种简便、无创且敏感的技术。

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本文引用的文献

1
Leishmaniasis and AIDS co-infection: the Spanish example.利什曼病与艾滋病合并感染：以西班牙为例。
Parasitol Today. 1994 Apr;10(4):160-3. doi: 10.1016/0169-4758(94)90270-4.
2
Diagnosis of visceral leishmaniasis in HIV-infected individuals using peripheral blood smears.利用外周血涂片对HIV感染者进行内脏利什曼病的诊断。
AIDS. 1993 Feb;7(2):227-30. doi: 10.1097/00002030-199302000-00011.
3
Leishmania donovani-reactive Th1- and Th2-like T-cell clones from individuals who have recovered from visceral leishmaniasis.来自内脏利什曼病康复个体的杜氏利什曼原虫反应性Th1样和Th2样T细胞克隆。
Infect Immun. 1993 Mar;61(3):1069-73. doi: 10.1128/iai.61.3.1069-1073.1993.
4
An easy and quick method for the diagnosis of visceral leishmaniasis in HIV-1-infected individuals.一种用于诊断HIV-1感染个体内脏利什曼病的简便快速方法。
AIDS. 1993 Oct;7(10):1399. doi: 10.1097/00002030-199310000-00023.
5
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J Clin Microbiol. 1994 Mar;32(3):746-9. doi: 10.1128/jcm.32.3.746-749.1994.
6
[Rheumatoid arthritis and hepatic nodular regenerative hyperplasia].[类风湿性关节炎与肝结节性再生性增生]
Rev Clin Esp. 1994 Jan;194(1):70.
7
Could infected drug-users be potential Leishmania infantum reservoirs?感染了的吸毒者会是婴儿利什曼原虫的潜在宿主吗？
AIDS. 1994 Jun;8(6):854. doi: 10.1097/00002030-199406000-00024.
8
Indirect xenodiagnosis of visceral leishmaniasis in 10 HIV-infected patients using colonized Phlebotomus perniciosus.使用定殖的白蛉对10例HIV感染患者进行内脏利什曼病的间接异种诊断。
AIDS. 1994 Feb;8(2):277-9. doi: 10.1097/00002030-199402000-00024.
9
Contribution of serological tests to the diagnosis of visceral leishmaniasis in patients infected with the human immunodeficiency virus.血清学检测对人类免疫缺陷病毒感染患者内脏利什曼病诊断的贡献。
Trans R Soc Trop Med Hyg. 1994 May-Jun;88(3):301-2. doi: 10.1016/0035-9203(94)90088-4.
10
Visceral leishmaniasis in immunocompromised hosts.免疫功能低下宿主中的内脏利什曼病
Am J Med. 1987 Dec;83(6):1098-102. doi: 10.1016/0002-9343(87)90948-x.