Martínez P, de la Vega E, Laguna F, Soriano V, Puente S, Moreno V, Sentchordi M J, García-Aguado C, González-Lahoz J
Sección de Hematología y Bioquímica, Instituto de Salud Carlos III, Madrid, Spain.
AIDS. 1993 Feb;7(2):227-30. doi: 10.1097/00002030-199302000-00011.
To compare the clinical and laboratory features of visceral leishmaniasis (kala-azar) in HIV-infected and non-infected subjects, and to determine the presence of Leishmania amastigotes in circulating leukocytes using peripheral blood smears.
Twenty-eight HIV-infected and six HIV-negative adult patients diagnosed as having kala-azar presenting at one institution over a 7-year period.
Retrospective review of clinical charts and re-examination of peripheral blood smears.
There were no significant differences in the clinical presentation and laboratory features of HIV-positive and HIV-negative patients. However, Leishmania amastigotes were observed in circulating leukocytes in eight out of the 17 available peripheral blood smears (15 from HIV-infected patients). All eight individuals presenting with Leishmania in peripheral blood leukocytes were HIV-positive.
Direct visualization of Leishmania amastigotes in leukocytes on peripheral blood smears enabled the diagnosis of kala-azar in a high proportion [eight out of 15 (53%)] of our HIV-infected patients.
比较HIV感染和未感染患者内脏利什曼病（黑热病）的临床和实验室特征，并通过外周血涂片确定循环白细胞中利什曼原虫无鞭毛体的存在情况。
28例HIV感染的成年患者和6例HIV阴性的成年患者，这些患者在7年期间于同一机构被诊断为患有黑热病。
回顾性查阅临床病历并重新检查外周血涂片。
HIV阳性和HIV阴性患者的临床表现和实验室特征无显著差异。然而，在17份可用的外周血涂片中，有8份（其中15份来自HIV感染患者）在循环白细胞中观察到利什曼原虫无鞭毛体。外周血白细胞中出现利什曼原虫的所有8人均为HIV阳性。
通过外周血涂片直接观察白细胞中的利什曼原虫无鞭毛体，使我们大部分（15例中的8例，占53%）HIV感染患者的黑热病得以诊断。
