Jontell M, Hanks C T, Bratel J, Bergenholtz G
Department of Endodontology and Oral Diagnosis, Faculty of Odontology, University of Göteborg, Sweden.
J Dent Res. 1995 May;74(5):1162-7. doi: 10.1177/00220345950740050401.
Monomeric resin components from dental composites are toxic to fibroblasts in culture and thus may interfere with the local immune system of the pulp, reducing its effective defense potential, either by cytotoxicity or by a more specific immune mechanism. Therefore, the present study was undertaken to observe the cytotoxic effects elicited by certain unpolymerized components of resin composites upon the function of accessory pulp cells in mitogen-induced proliferation of T-lymphocytes. Accessory cells from the rat incisor pulp were released following enzymatic digestion with collagenase. The assay included incubation of these cells with purified T-lymphocytes from cervical lymph nodes for 72 h in the presence of different concentrations of the resin components. The proliferative T-lymphocyte response was monitored by 3H-thymidine incorporation. Initially, we conducted experiments on spleen cells to determine the proper concentration intervals for suitable testing of the resin components. To assess the individual susceptibility of accessory cells and T-lymphocytes, we pre-treated each of these cells with some of the test materials prior to assay. At low concentrations, urethane dimethacrylate (UDMA), bisglycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and bis-phenol A (BPA) increased spleen cell proliferation to concanavalin A (con A). Purified T-lymphocytes stimulated by pulpal cells did not show enhanced responses to UDMA, bis-GMA, glycidyl mehtacrylate (GMA), or N,N,-dihydroxyethyl-p-toluidine (DHEpT). At higher concentrations, all substances except camphoroquinone (CAMP) showed inhibitory effects in both test systems. The in vitro study shows that resin components can evoke either immunosuppression or immunostimulation on mitogen-driven proliferation of purified T-lyumphocytes and spleen cells.
牙科复合材料中的单体树脂成分对培养中的成纤维细胞有毒性，因此可能会干扰牙髓的局部免疫系统，通过细胞毒性或更特殊的免疫机制降低其有效的防御潜能。因此，本研究旨在观察树脂复合材料的某些未聚合成分对牙髓辅助细胞在丝裂原诱导的T淋巴细胞增殖功能上所引发的细胞毒性作用。用胶原酶进行酶消化后，从大鼠切牙髓中释放出辅助细胞。该试验包括在不同浓度的树脂成分存在下，将这些细胞与来自颈淋巴结的纯化T淋巴细胞孵育72小时。通过掺入3H-胸腺嘧啶核苷监测增殖性T淋巴细胞反应。最初，我们对脾细胞进行实验以确定对树脂成分进行适当测试的合适浓度范围。为了评估辅助细胞和T淋巴细胞的个体敏感性，我们在试验前用一些测试材料对这些细胞中的每一种进行预处理。在低浓度下，聚氨酯二甲基丙烯酸酯（UDMA）、双甲基丙烯酸缩水甘油酯（bis-GMA）、三乙二醇二甲基丙烯酸酯（TEGDMA）和双酚A（BPA）可增加脾细胞对刀豆球蛋白A（Con A）的增殖。牙髓细胞刺激的纯化T淋巴细胞对UDMA、bis-GMA、甲基丙烯酸缩水甘油酯（GMA）或N，N-二羟乙基对甲苯胺（DHEpT）未显示出增强的反应。在较高浓度下，除樟脑醌（CAMP）外的所有物质在两个测试系统中均显示出抑制作用。体外研究表明，树脂成分可对纯化的T淋巴细胞和脾细胞的丝裂原驱动增殖引发免疫抑制或免疫刺激。
