Makar T K, Rimpel-Lamhaouar K, Abraham D G, Gokhale V S, Cooper A J
Department of Biochemistry, Cornell University Medical College, New York, NY 10021, USA.
J Neurochem. 1995 Jul;65(1):287-91. doi: 10.1046/j.1471-4159.1995.65010287.x.
The specific activities of superoxide dismutase, catalase, and glutathione S-transferase (mu subtype) were significantly lower in the brains of mice with type II diabetes than in the brains of control mice. On the other hand, the specific activity of glutathione peroxidase was unaltered. The concentration of vitamin E, but not that of total glutathione and ascorbate, was increased in the brains of the type II diabetic mice. The relative amount of polyunsaturated fatty acids (as determined with soybean lipoxygenase) was increased in whole brains and crude synaptosomal membranes of the type II diabetic mice. Endogenous levels of thiobarbituric acid-positive material were decreased in both whole brain homogenates and crude synaptosomal membranes of the db/db mice. Susceptibility of lipids within whole brain homogenates and crude synaptosomal membranes of mice with type II diabetes to peroxidation with iron/ascorbate was also markedly decreased compared with that of controls. Vitamin E is known to quench lipid peroxidation. Therefore, decreased lipid peroxidation in the type II mouse brain may be due to increased vitamin E content.
与对照小鼠的大脑相比，II型糖尿病小鼠大脑中超氧化物歧化酶、过氧化氢酶和谷胱甘肽S-转移酶（μ亚型）的比活性显著降低。另一方面，谷胱甘肽过氧化物酶的比活性未改变。II型糖尿病小鼠大脑中维生素E的浓度升高，而总谷胱甘肽和抗坏血酸的浓度未升高。II型糖尿病小鼠全脑和粗制突触体膜中多不饱和脂肪酸的相对含量（用大豆脂氧合酶测定）增加。db/db小鼠全脑匀浆和粗制突触体膜中硫代巴比妥酸阳性物质的内源性水平均降低。与对照组相比，II型糖尿病小鼠全脑匀浆和粗制突触体膜中的脂质对铁/抗坏血酸过氧化的敏感性也显著降低。已知维生素E可淬灭脂质过氧化。因此，II型小鼠大脑中脂质过氧化的降低可能是由于维生素E含量增加所致。
