Ofner D, Riedmann B, Maier H, Hittmair A, Rumer A, Tötsch M, Spechtenhauser B, Böcker W, Schmid K W
Gerhard-Domagk-Institute of Pathology, University of Münster, Germany.
J Pathol. 1995 Apr;175(4):441-8. doi: 10.1002/path.1711750412.
Quantification of silver-stained nucleolar organizer region associated proteins (AgNORs) was introduced in histopathology as a marker of cellular and nucleolar activity. However, due to the poor staining quality obtained on routinely processed archival material, the method yielded controversial and sometimes non-reproducible results. The recent introduction of wet autoclave pretreatment has reliably improved AgNOR staining quality on routinely formalin-fixed and paraffin-embedded tissues. In the present study, 92 routinely processed colorectal carcinomas were investigated, applying this novel staining technique. Subsequent standardized morphometric analysis revealed, irrespective of common tumour staging or grading classifications, a statistically highly significant correlation between AgNOR parameters and clinical course. The usefulness of standardized AgNOR parameters for the independent prediction of patient survival was proven by uni- and multivariate analysis.
银染核仁组织区相关蛋白（AgNORs）的定量分析作为细胞和核仁活性的标志物被引入组织病理学。然而，由于常规处理的存档材料染色质量较差，该方法产生了有争议的结果，有时甚至不可重复。最近采用的湿热高压预处理可靠地提高了常规福尔马林固定石蜡包埋组织上AgNOR的染色质量。在本研究中，应用这种新的染色技术对92例常规处理的结肠直肠癌进行了研究。随后的标准化形态计量分析显示，无论常见的肿瘤分期或分级分类如何，AgNOR参数与临床病程之间在统计学上具有高度显著的相关性。单因素和多因素分析证明了标准化AgNOR参数对独立预测患者生存的有用性。
