Sood S K, Zemel L S, Ilowite N T
Pediatric Lyme Disease Center, Schneider Children's Hospital, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, NY 11040, USA.
J Rheumatol. 1995 Apr;22(4):758-61.
To determine the pattern of bands present on an immunoblot (Western blot) in children with Lyme arthritis.
Sera from 20 children with Lyme arthritis from an endemic area were assayed, and the results compared with those obtained in 162 control sera from the same area. The study was retrospective, serum bank based.
Eighty-seven percent of control sera revealed no bands on immunoblot, and all had < or = 4 bands. All Lyme arthritis sera revealed > or = 5 bands (mean 8.4, range 5-13 bands). Bands of molecular weight 25, 28, 39, 47, 50, and 93 kDa were seen in patients and not in controls. There was no correlation between duration of arthritis or activity of arthritis at presentation and number of bands present.
Criteria were derived for a positive immunoblot based on the number and molecular weights of bands seen in our laboratory. These are the presence of 5 or more bands, of which at least one is an apparently specific band (25, 28, 39, 47, 50, or 93 kDa). Results of immunoblot are useful to confirm clinically suspected Lyme arthritis.
确定莱姆关节炎患儿免疫印迹法（蛋白质印迹法）上出现的条带模式。
检测了来自流行地区的20例莱姆关节炎患儿的血清，并将结果与来自同一地区的162份对照血清的结果进行比较。该研究是基于血清库的回顾性研究。
87%的对照血清在免疫印迹上未显示条带，且所有对照血清的条带数均≤4条。所有莱姆关节炎血清均显示≥5条带（平均8.4条，范围为5 - 13条）。分子量为25、28、39、47、50和93 kDa的条带出现在患者血清中，而对照血清中未出现。关节炎持续时间或就诊时关节炎的活动度与出现的条带数之间无相关性。
根据我们实验室观察到的条带数量和分子量得出了免疫印迹阳性的标准。这些标准是出现5条或更多条带，其中至少有一条是明显的特异性条带（25、28、39、47、50或93 kDa）。免疫印迹结果有助于确诊临床疑似的莱姆关节炎。
